文献类型： 外文期刊

作者： Zhu, Zaobing ¹ ; Yang, Litao ¹ ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab & Dev Sci, Shanghai 200240, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China

3.Shanghai Jiao Tong Univ, Yazhou Bay Inst Deepsea Scitech, Sanya 572024, Peoples R China

关键词： Rolling circle amplification; CIRPSR/Cas systems; Target diagnosis

期刊名称：TRAC-TRENDS IN ANALYTICAL CHEMISTRY （ 影响因子：11.8； 五年影响因子：12.7 ）

ISSN： 0165-9936

年卷期： 2024 年 180 卷

页码：

收录情况： SCI

摘要： The fusion of Rolling Circle Amplification (RCA) and the Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-associated proteins (CRISPR-Cas) stands at the cutting edge of molecular diagnostics, offering a potent tool for the targeted investigation of genetic material. RCA, known for its robust isothermal amplification capabilities, enables the exponential replication of circular nucleic acid templates. The integration of RCA with CRISPR-Cas technologies has paved the way for innovative diagnostic strategies that boast remarkable specificity and sensitivity in the detection of nucleic acid sequences. This review outlines the fundamental aspects of RCA, including its underlying principles, the diversity of its methodologies, and its variants. We provide a comprehensive overview of the latest advancements in the design, development, and practical application of RCA biosensors that employ the CRISPR/Cas system for enhanced biosensing capabilities. The review further explores the broad potential of RCA in applications beyond traditional biological targets, such as protein and biomarker detection. We conclude with an analytical discussion on the present challenges and outlook of RCA-CRISPR/Casbased diagnostics, underscoring the transformative impact of these technologies on the various fields of molecular diagnostics.