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A novel electrochemical aptasensor for ultrasensitive detection of herbicide prometryn based on its highly specific aptamer and Ag@Au nanoflowers

文献类型： 外文期刊

作者： Zhang, Zhenzhong 1 ; Luan, Yunxia 2 ; Ru, Shaoguo 1 ; Teng, Hayan 1 ; Li, Yuejiao 1 ; Liu, Minhao 1 ; Wang, Jun 1 ;

作者机构： 1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Qual Stand & Testing Technol, Beijing 100097, Peoples R China

3.Ocean Univ China, Coll Marine Life Sci, 5 Yushan Rd, Qingdao 266003, Shandong, Peoples R China

关键词： Prometryn; Aptasensor; Capture-SELEX; Ag@Au NFs

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.4 ）

ISSN： 0039-9140

年卷期： 2023 年 265 卷

页码：

收录情况： SCI

摘要： Herbicide prometryn has become a common pollutant in aquatic environments and caused adverse impacts on ecosystems. This study developed an ultrasensitive electrochemical aptasensor for prometryn based on its highly affinitive and specific aptamer and Ag@Au nanoflowers (Ag@AuNFs) for signal amplification. Firstly, this study improved the Capture-SELEX strategy to screen aptamers and obtained aptamer P60-1, which had a high affinity (Kd: 23 nM) and could distinguish prometryn from its structural analogues. Moreover, the typical stem-loop structure in aptamer P60-1 was found to be the binding pocket for prometryn. Subsequently, an electro-chemical aptasensor for prometryn was established using multiwalled carbon nanotubes and reduced graphene oxide as electrode substrate, Ag@Au NFs as signal amplification element, and aptamer P60-1 as recognition element. The aptasensor had a detection range of 0.16-500 ng/mL and a detection limit of 60 pg/mL, which was much lower than those of existing detection methods. The aptasensor had high stability and good repeatability, and could specifically detecting prometryn. Furthermore, the utility of the aptasensor was validated by measuring prometryn in environmental and biological components. Therefore, this study provides a robust and ultrasensitive aptasensor for accurate detection for prometryn pollution.

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