文献类型： 外文期刊
作者： Cai, Shufang 1 ; Hu, Bin 2 ; Wang, Xiaoyu 1 ; Liu, Tongni 3 ; Lin, Zhuhu 1 ; Tong, Xian 1 ; Xu, Rong 1 ; Chen, Meilin 1 ; Duo, Tianqi 1 ; Zhu, Qi 1 ; Liang, Ziyun 1 ; Li, Enru 1 ; Chen, Yaosheng 1 ; Li, Jianhao 2 ; Liu, Xiaohong 1 ; Mo, Delin 1 ;
作者机构： 1.Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510006, Guangdong, Peoples R China
2.Guangdong Acad Agr Sci, Inst Anim Sci, Guangdong Key Lab Anim Breeding & Nutr, State Key Lab Livestock & Poultry Breeding, Guangzhou 510640, Guangdong, Peoples R China
3.Univ British Columbia, Fac Forestry, Vancouver, BC V6T 1Z4, Canada
关键词： Pig; Myogenic differentiation; Skeletal muscle; scRNA-seq; scATAC-seq
期刊名称：BMC BIOLOGY （ 影响因子：5.4； 五年影响因子：7.1 ）
ISSN：
年卷期： 2023 年 21 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundSkeletal muscle development is a multistep process whose understanding is central in a broad range of fields and applications, from the potential medical value to human society, to its economic value associated with improvement of agricultural animals. Skeletal muscle initiates in the somites, with muscle precursor cells generated in the dermomyotome and dermomyotome-derived myotome before muscle differentiation ensues, a developmentally regulated process that is well characterized in model organisms. However, the regulation of skeletal muscle ontogeny during embryonic development remains poorly defined in farm animals, for instance in pig. Here, we profiled gene expression and chromatin accessibility in developing pig somites and myotomes at single-cell resolution.ResultsWe identified myogenic cells and other cell types and constructed a differentiation trajectory of pig skeletal muscle ontogeny. Along this trajectory, the dynamic changes in gene expression and chromatin accessibility coincided with the activities of distinct cell type-specific transcription factors. Some novel genes upregulated along the differentiation trajectory showed higher expression levels in muscular dystrophy mice than that in healthy mice, suggesting their involvement in myogenesis. Integrative analysis of chromatin accessibility, gene expression data, and in vitro experiments identified EGR1 and RHOB as critical regulators of pig embryonic myogenesis.ConclusionsCollectively, our results enhance our understanding of the molecular and cellular dynamics in pig embryonic myogenesis and offer a high-quality resource for the further study of pig skeletal muscle development and human muscle disease.
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