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Optimization of Heat-Resistance Technology for a Duck Hepatitis Lyophilized Live Vaccine

文献类型： 外文期刊

作者： Zhao, Yanhong 1 ; Deng, Bihua 1 ; Pan, Xiaoqing 2 ; Zhang, Jinqiu 1 ; Zuo, Xiaoxin 1 ; Wang, Junning 1 ; Lv, Fang 1 ; Lu, Yu 1 ; Hou, Jibo 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Natl Res Ctr Engn & Technol Vet Biol, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr Sci, Livestock Res Inst, Nanjing 210014, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

4.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Peoples R China

5.Jiangsu Univ, Sch Pharm, Zhenjiang 212013, Peoples R China

关键词： DHV; optimization of the freeze-drying process; stabilizers; thermostability

期刊名称：VACCINES （ 影响因子：4.961； 五年影响因子：5.325 ）

ISSN：

年卷期： 2022 年 10 卷 2 期

页码：

收录情况： SCI

摘要： In this study, to improve the quality of a live attenuated vaccine for duck viral hepatitis (DHV), the lyophilization of a heat-resistant duck hepatitis virus vaccine was optimized. The optimized heat protectors were made of 10% sucrose, 1.2% pullulan, 0.5% PVP, and 1% arginine, etc., with a titer freeze-drying loss of <= 0.50 Lg. The vaccine product's valence measurements demonstrated the following: the vaccine could be stored at 2-8 degrees C for 18 months with a virus titer loss <= 0.91 Lg; at 37 degrees C for 10 days with a virus valence loss <= 0.89 Lg; and at 45 degrees C for 3 days with a virus titer loss <= 0.90 Lg. Regarding safety, no deaths occurred in two-day-old ducklings immunized with a 10 times dose vaccine; their energy, diet, and weight gain were all normal, demonstrating that the DHV heat-resistant vaccines were safe for ducklings and did not cause any immune side effects. Duck viral hepatitis freeze-dried vaccine began to produce antibodies at 7 d after immunization, reached above 5.0 on 14 d, and reached above 7.0 on 21 d, showing a continuous upward trend. This indicates that duck viral hepatitis vaccine has a good immunogen level. The optimization of the freeze-drying process saves costs and also improves the quality of the freeze-drying products, which provides important theoretical and technical support for the further study of vaccine products.

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