An Improved GC-MS Method for Malondialdehyde (MDA) Detection: Avoiding the Effects of Nitrite in Foods
文献类型： 外文期刊
作者： Wang, Wenjie 1 ; Zhang, Zhiwen 1 ; Liu, Xiaoying 1 ; Cao, Xiaoji 5 ; Wang, Lianzhu 6 ; Ding, Yuting 1 ; Zhou, Xuxia 1 ;
作者机构： 1.Zhejiang Univ Technol, Coll Food Sci & Technol, Hangzhou 310014, Peoples R China
2.Key Lab Marine Fishery Resources Exploitment Util, Hangzhou 310014, Peoples R China
3.Natl R&D Branch Ctr Pelag Aquat Prod Proc Hangzh, Hangzhou 310014, Peoples R China
4.Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China
5.Zhejiang Univ Technol, Res Ctr Anal & Measurement, Hangzhou 310014, Peoples R China
6.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
关键词： malondialdehyde; lipid peroxidation; pickled products; nitrite; GC-MS
期刊名称：FOODS （ 影响因子：5.561； 五年影响因子：5.94 ）
ISSN：
年卷期： 2022 年 11 卷 9 期
页码：
收录情况： SCI
摘要： Malondialdehyde (MDA) is one of the representative end products under lipid peroxidation, indicating the degree of lipid oxidation in foods. However, compounds in pickled products, especially the nitrite in salted lean pork can react with MDA under the acidic condition, leads to the loss of MDA and an underestimation on lipid oxidation through the conventional assay. In this study, the quantification for MDA in the sample containing sodium nitrite were found lacking accuracy by the thiobarbituric acid (TBA) assay and chromatography assay based on alkaline hydrolysis as the reaction between them were difficult to be completely inhibited. Among other trials, the improvement GC-MS analysis utilizing deuterium substituted MDA (MDA-d(2)) as an internal standard and applying perfluorophenylhydrazine (PFPH) as a derivative reagent can reduce the deviations from the presence of nitrite in the salted lean pork meat and the recovery is between 93.9% and 98.4% and coefficient of variation for the intermediate precision is between 1.1 and 3.5% using the method. The advanced gas chromatograph mass spectrometer (GC-MS) assay also has a very low detection limit (0.25 ng/mL) with both hydrolysis types.
- 相关文献
作者其他论文 更多>>
-
Oyster-derived peptides as pancreatic lipase inhibitors: Kinetics, molecular interactions, and gastrointestinal stability
作者：Chen, Hui;Dong, Xiting;Zhou, Xuxia;Zhou, Mengyuan;Xu, Zhihao;Chen, Hui;Zhao, Yongqiang;Chen, Hui;Dong, Xiting;Zhou, Xuxia;Zhou, Mengyuan;Xu, Zhihao;Chen, Hui;Dong, Xiting;Zhou, Xuxia;Zhou, Mengyuan;Xu, Zhihao;Chen, Hui;Xu, Zhe
关键词：Oyster peptide; pancreatic lipase; inhibition mechanism; Molecular docking
-
Inclusion of macroalgae in the diet - a comparative survey from Norway, Chile and China
作者：Goecke, Franz;Ergon, Ashild;Aakre, Inger;Angarita, Lisse;Escobar, Maria Cristina;Cisternas, Silvana;Li, Na;Wang, Lianzhu;Li, Xiaodong;Pang, Shaojun
关键词：seaweed; nutrition; seafood; iodine; food risks; culinary traditions
-
Development of a Real-Time PCR Method for Identifying Four Major Freshwater Fish in Marine Fish Surimi
作者：Li, Yutong;Li, Yutong;Qu, Meng;Liu, Enchong;Jiang, Yanhua;Guo, Yingying;Zhu, Wenjia;Li, Na;Wang, Lianzhu;Yao, Lin;Liu, Enchong;Wan, Shuo
关键词：Marine fish surimi; Freshwater fish; Identification; ND5 gene; QPCR
-
Elemental profiling of red seaweed Neopyropia yezoensis used in fast authenticating the geographical origin and food safety assessment
作者：Zhao, Yanfang;Kang, Xuming;Guo, Yingying;Wang, Lianzhu;Sheng, Xiaofeng;Tan, Zhijun;Wu, Jifa;Tan, Zhijun;Tan, Zhijun
关键词：Food analysis; Food composition; Heavy metals; Traceability; Red seaweed
-
Development of Single-Nucleotide Polymorphism (SNP)-Based Species-Specific Real-Time PCR Assays for Authenticating Five Highly Priced Tuna
作者：Qu, Meng;Jiang, Yanhua;Li, Na;Guo, Yingying;Zhu, Wenjia;Li, Na;Zhao, Xinnan;Yao, Lin;Wang, Lianzhu;Li, Na;Li, Na
关键词：tuna; species identification; 2b-RAD; SNP; real-time PCR
-
Evaluation of the Bioavailability of Iodine and Arsenic in Raw and Cooked Saccharina japonica Based on Simulated Digestion/Caco-2 Cell Model
作者：Li, Na;Geng, Zhaomeng;Guo, Yingying;Dai, Xinyue;Zhu, Wenjia;Yao, Lin;Jiang, Yanhua;Wang, Xiaojuan;Wang, Lianzhu;Geng, Zhaomeng;Dai, Xinyue;Dong, Hao;Wang, Huijie
关键词：Saccharina japonica; bioaccessibility; bioavailability; iodine; arsenic
-
Development and Evaluation of Real-Time Polymerase Chain Reaction (PCR) to Authenticate Sablefish (Anoplopoma fimbria) in Commercial Seafood Products
作者：Qu, Meng;Jiang, Yanhua;Guo, Yingying;Zhu, Wenjia;Li, Na;Li, Fengling;Yao, Lin;Tan, Zhijun;Wang, Lianzhu;Qu, Meng;Jiang, Yanhua;Guo, Yingying;Zhu, Wenjia;Liu, Shufang;Li, Na;Li, Fengling;Yao, Lin;Tan, Zhijun;Wang, Lianzhu;Liu, Shufang;Yao, Lin
关键词：Sablefish; real-time polymerase chain reaction; authentication; cytochrome b gene
