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Impact of Quorum Sensing Signal N-3-Oxododecanoyl Homoserine Lactone on Intestinal Porcine Enterocyte Cell

文献类型： 外文期刊

作者： Yang, Yang 1 ; Wang, Congrong 1 ; Liu, Yun 1 ; Ma, Xinyan 1 ; Xia, Pengpeng 1 ; Zhou, Mingxu 4 ; Duan, Qiangde 1 ; Zhu, Guoqiang 1 ;

作者机构： 1.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China

2.Jiangsu Coinnovat Ctr Important Anim Infect Dis &, Yangzhou 225009, Peoples R China

3.Jiangsu Higher Educ Inst, Joint Lab Int Cooperat Prevent & Control Technol I, Yangzhou 225009, Peoples R China

4.Jiangsu Acad Agr Sci, Natl Res Ctr Engn & Technol Vet Biol, Inst Vet Immunol & Engn, Nanjing 210014, Peoples R China

5.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Peoples R China

关键词： Quorum Sensing; 3OC12-HSL; N-3-Oxododecanoyl Homoserine Lactone (OdDHL); Hexanoyl-L-Homoserine Lactone (C6-HSL); Epithelial Cells in the Small Intestine of Piglets; Biochemicals

期刊名称：JOURNAL OF BIOMEDICAL NANOTECHNOLOGY （ 影响因子：2.9； 五年影响因子：3.2 ）

ISSN： 1550-7033

年卷期： 2023 年 19 卷 8 期

页码：

收录情况： SCI

摘要： Quorum sensing (QS) is an autoinducer (AI) molecules mediated signaling system in bacteria. It is generally believed that bacteria can regulate their own characteristics or virulence through OS. This study investigated the direct effects of a quorum sensing signal molecule N-3-oxododecanoyl homoserine lactone (3OC12-HSL, synonyms: OdDHL) on host cells, to further reveal the function of QS system in the interaction between bacteria and host cells. Intestinal porcine enterocyte IP: 203 8 10920 On: Wed 23 Aug 2023 14:13:06 cell line (IPEC-J2) were used as model cells, and the AHL signal molecle OdDHL was used to stimulate the cells to Copyright: American Scientific Pub ishers observe the effect of quorum sensing signals on hostDeliveredcells. byTheIngenaresults showed that OdDHL had an obvious effect on the morphology of IPEC-J2 cells, and a lower concentration and shorter time of stimulation could induce cell shrinkage and rounding. MTT assay revealed that OdDHL had no effect on IPEC-J2 cell viability, suggesting that the cell morpho-logical changes were not from signaling molecule toxicity. Using different concentrations and different exposure times of OdDHL to stimulate IPEC-J2 cells, we demonstrated that changes in cell morphology were positively correlated with the concentration of AHL and exposure time. Bacterial adhesion assay showed that OdDHL could enhance the adhesion of enteropathogenic E. coli to host cell IPEC-J2, while reducing its invasion into host cell. Fluorescent quantitative PCR and Western blot revealed that the expression of tight junction protein ZO-1 was significantly increased. The expression levels of multiple genes that related to cytoskeletons and cell junction were all regulated by OdDHL. In conclusion, OdDHL could directly affect the biological characteristics of IPEC-J2 cells such as morphology and tight junction, reduce the intestinal defences of hosts, thereby promoting the adhesion and injury of pathogenic bacteria to the intestine of piglets. This study provides a basic reference for further understanding of the pathogenic role of regulating pathogenic bacterial populations and their prevention against diseases.

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