您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Functional Characterization of Galectin-8 from Golden Pompano Trachinotus ovatus Reveals Its Broad-Spectrum Antimicrobial Activity

文献类型： 外文期刊

作者： Pan, Jin-Min 1 ; Gao, Jie 1 ; Liu, Ming Jian 1 ; Zhu, Ke-Cheng 1 ; Guo, Hua-Yang 1 ; Liu, Bao-Suo 1 ; Zhang, Nan 1 ; Zhang, Dian-Chang 1 ;

作者机构： 1.Sanya Trop Fisheriers Res Inst, Sanya 572108, Hainan, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab South China Sea Fishery Resources Expoitat, 231 Xingang Rd West, Guangzhou 510300, Guangdong, Peoples R China

3.Guangdong Prov Engineer Technol Res Ctr Marine Bio, Guangzhou, Guangdong, Peoples R China

4.Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510006, Peoples R China

关键词： Galectin-8; Antibacterial activity; Trachinotus ovatus; Streptococcus agalactiae

期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：2.8； 五年影响因子：3.0 ）

ISSN： 1436-2228

年卷期： 2025 年 27 卷 1 期

页码：

收录情况： SCI

摘要： Galectins exhibit a variety of biological functions through interactions with their ligands, including galactose and its derivatives. Tandem-repeat galectins, such as Galectin-8, can act as pattern recognition receptors to aggregate and neutralize bacterial pathogens. In this study, Galectin-8 was identified in Trachinotus ovatus (golden pompano). Galectin-8 consists of two carbohydrate recognition domains (CRDs) connected by a linker region. Furthermore, molecular docking analysis suggests that the C-terminal CRD can bind galactose, mannose, and N-acetylglucosamine at similar binding sites. ToGal-8 expression levels were highest in the brain and blood of healthy T. ovatus. However, following infection with Streptococcus agalactiae, expression levels in the spleen and head kidney surged at 48 h, while liver expression significantly decreased by 96 h. Cytoplasmic Galectin-8 expression was upregulated after stimulation by peptidoglycan compared with lipopolysaccharide. Recombinant ToGal-8 (rToGal-8) was produced using a prokaryotic expression system. This protein could agglutinate red blood cells from rabbits, carp, and T. ovatus independently of Ca2+. Moreover, it was also effective in aggregating and eliminating several bacterial strains, such as Staphylococcus aureus, Bacillus subtilis, Vibrio vulnificus, S. agalactiae, Pseudomonas aeruginosa, and Aeromonas hydrophila. Therefore, this study provides an in-depth analysis of the function of T. ovatus Galectin-8 for the first time, offering guidance for the healthy aquaculture of T. ovatus.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台