Role of animal pole protuberance and microtubules during meiosis in sea cucumber Apostichopus japonicus oocytes
文献类型: 外文期刊
作者: Pang Zhenguo 3 ; Chang Yaqing 1 ; Sun Huiling 2 ; Yu Jiaping 1 ;
作者机构: 1.Dalian Ocean Univ, Minist Agr, Key Lab Marine Culture, Dalian 116023, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fishery Res Inst, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
关键词: Apostichopus japonicus;oocyte protuberance;oocyte maturation;microtubule organizing center;polar body formation
期刊名称:CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY ( 影响因子:1.068; 五年影响因子:0.983 )
ISSN: 0254-4059
年卷期: 2010 年 28 卷 3 期
页码:
收录情况: SCI
摘要: Fully grown oocytes of Apostichopus japonicus have a cytoplasmic protuberance where the oocyte attaches to the follicle. The protuberance and the oolamina located on the opposite side of the oocyte indicate the animal-vegetal axis. Two pre-meiotic centrosomes are anchored to the protuberance by microtubules between centrosomes and protuberance. After meiosis reinitiation induced by DTT solution, the germinal vesicle (GV) migrates towards the protuberance. The GV breaks down after it migrates to the oocyte membrane on the protuberance side. The protuberance then contracts back into the oocyte and the first polar body extrudes from the site of the former protuberance. The second polar body forms beneath the first. Thus the oocyte protuberance indicates the presumptive animal pole well before maturation of the oocyte.
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