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Lectin Receptor-Like Protein Kinase OsNRFG6 is Required for Embryo Sac Development and Fertilization in Neo-Tetraploid Rice

文献类型： 外文期刊

作者： Zhao, Chongchong 1 ; Li, Qihang 1 ; Ge, Qi 1 ; Chen, Rou 1 ; Yu, Hang 2 ; Wu, Jinwen 1 ; Liu, Xiangdong 1 ; Lu, Zijun 1 ;

作者机构： 1.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agro Bi, Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China

2.Guangdong Acad Agr Sci, Rice Res Inst, Guangzhou 510642, Peoples R China

3.South China Agr Univ, Guangdong Prov Key Lab Plant Mol Breeding, Guangzhou 510642, Peoples R China

4.South China Agr Univ, Coll Agr, Guangdong Base Bank Lingnan Rice Germplasm Resourc, Guangzhou 510642, Peoples R China

关键词： Lectin receptor-like Kinase (LecRLK); Neo-tetraploid rice; Fertility; Embryo sac; Double fertilization

期刊名称：RICE （ 影响因子：4.8； 五年影响因子：5.4 ）

ISSN： 1939-8425

年卷期： 2024 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Great yield-enhancing prospects of autotetraploid rice was restricted by various polyploidy-induced reproductive dysfunction. To surmount these challenges, our group has generated a series of valuable fertile tetraploid lines (denoted as neo-tetraploid rice) through 20-year efforts. With this context, a G-type lectin receptor-like kinase, OsNRFG6, was identified as a pivotal factor associated with reproductive regulation in neo-tetraploid rice. Nevertheless, it is still elusive about a comprehensive understanding of its precise functional roles and underlying molecular mechanisms during reproduction of neo-tetraploid rice. Here, we demonstrated that OsNRFG6 executed a constitutive expression pattern and encoded proteins localizing in perinucleus and endoplasmic reticulum. Subsequently, four independent mutant lines of OsNRFG6 within neo-tetraploid rice background were further identified, all displaying low seed-setting rate due to abortive embryo sacs and defective double fertilization. RNA-seq and RT-qPCR revealed a significant down-regulation of OsNRFG6 and female reproductive genes such as OsMEL1 and LOG in ovaries prior to and post-fertilization, attributing this effect to OsNRFG6 mutation. Furthermore, through yeast-two hybrids, bimolecular fluorescence complementation assays, and luciferase complementation imaging assays, it was determined that OsNRFG6 could interact with itself and two female reproductive proteins (LOG and OsDES1) to form protein complexes. These results elucidate the reproductive functions and molecular pathway governed by OsNRFG6 in regulating fertility of neo-tetraploid rice, offering insights into molecular understanding of fertility improvement in polyploid rice.

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