文献类型： 外文期刊

作者： Iqbal, Mudassar ¹ ; Waqas, Muhammad ³ ; Mo, Quan ¹ ; Shahzad, Muhammad ² ; Zeng, Zhibo ¹ ; Qamar, Hammad ⁴ ; Mehmood, Khalid ² ; Kulyar, Muhammad Fakhar-e-Alam ¹ ; Nawaz, Shah ¹ ; Li, Jiakui ¹ ;

作者机构： 1.Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Peoples R China

2.Islamia Univ Bahawalpur, Fac Vet & Anim Sci, Bahawalpur 63100, Pakistan

3.Univ Poonch Rawalakot, Fac Vet & Anim Sci, Dept Vet Clin Sci, Poonch 12350, Azad Jammu & Ka, Pakistan

4.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou 510640, Peoples R China

关键词： Apoptosis; Baicalin; Chicken; Thiram; Tibial dyschondroplasia; Toxicity

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2023 年 268 卷

页码：

收录情况： SCI

摘要： Avian tibial dyschondroplasia (TD) is a skeletal disease affecting fast growing chickens, resulting in non-mineralized avascular cartilage. This metabolic disorder is characterized by lameness and reduced growth performance causing economic losses. The aim of this study was to investigate the protective effects of baicalin against TD caused by thiram exposure. A total of two hundred and forty (n = 240) one day-old broiler chickens were uniformly and randomly allocated into three different groups (n = 80) viz. control, TD, and baicalin groups. All chickens received standard feed, however, to induce TD, the TD and baicalin groups received thiram (tetramethylthiuram disulfide) at a rate of 50 mg/kg feed from days 4-7. The thiram induction in TD and baicalin groups resulted in lameness, high mortality, and enlarged growth-plate, poor production performance, reduction in ALP, GSH-Px, SOD, and T-AOC levels, and increased AST and ALT, and MDA levels. Furthermore, histopathological results showed less vascularization, and mRNA and protein expression levels of Sox-9, Col-II, and Bcl-2 showed significant downward trend, while caspase-9 displayed significant up-regulation in TD-affected chickens. After the TD induction, the baicalin group was orally administered with baicalin at a rate of 200 mg/kg from days 8-18. Baicalin administration increased the vascularization, and chondrocytes with intact nuclei, alleviated lameness, decreased GP size, increased productive capacity, and restored the liver antioxidant enzymes and serum biochemical levels. Furthermore, baicalin significantly up-regulated the gene and protein expressions of Sox-9, Col-II, and Bcl-2, and significantly down-regulated the expression of caspase-9 (p < 0.05). Therefore, the obtained results suggest that baicalin could be a possible choice in thiram toxicity alleviation by regulating apoptosis and chondrocyte proliferation in thiram-induced tibial dyschondroplasia.