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Production of cellulase by Aspergillus niger through fermentation of spent mushroom substance: Glucose inhibition and elimination approaches

文献类型： 外文期刊

作者： Ma, Xiaoyu 1 ; Gao, Ming 1 ; Li, Yuan 1 ; Wang, Qunhui 1 ; Sun, Xiaohong 3 ;

作者机构： 1.Univ Sci & Technol Beijing, Sch Energy & Environm Engn, Dept Environm Sci & Engn, 30 Xueyuan Rd, Beijing, Peoples R China

2.Univ Sci & Technol Beijing, Beijing Key Lab Resource oriented Treatment Ind Po, 30 Xueyuan Rd, Beijing, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China

关键词： Aspergillus niger cellulase; Spent mushroom substance; Glucose; Products inhibition; Thermodynamic analysis

期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：4.885； 五年影响因子：4.43 ）

ISSN： 1359-5113

年卷期： 2022 年 122 卷

页码：

收录情况： SCI

摘要： In this study, the best activity of Aspergillus niger cellulase produced from spent mushroom substance (SMS) was 18.82 U/mL, and its enzymatic hydrolysis ability was similar to that of commercial cellulase (120.70 U/g) at the same enzyme loading. Moreover, A. niger cellulase remained stable at pH and temperature ranges of 4.0-8.0 and 40-60 celcius, respectively. Subsequently, the feedback inhibition by glucose on A. niger cellulase was analysed. When the glucose concentration reached 30 g/L, the activation energy of denaturation was 10.69% lower than that of the group with 0 g/L glucose, indicating that 30 g/L of glucose worsened the stability and decreased the activity of the enzyme. However, the kinetic results showed that glucose inhibition of A. niger cellulase was reversible. A. niger cellulase was used in the lactic acid (LA) fermentation of SMS. The activity of A. niger cellulase inhibited by sugars was recovered through sugar consumption by lactic acid bacteria in separate hydrolysis and fermentation, and the final saccharification yield increased from 81.04% to 87.71%. Under simultaneous saccharification and fermentation, the inhibition was avoided because the sugar concentration was < 10 g/L. Therefore, the final LA concentration and productivity were 31.98 g/L and 0.89 g/L/h, respectively.

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