Isolation and characterization of polymorphic microsatellite loci from black snapper Sebastodes fuscescens (Houttuyn)
文献类型: 外文期刊
作者: Yang, Jing-Feng 1 ; Zhao, Li-Li 1 ; Shao, Chang-Wei 1 ; Ji, Xiang-Shan 1 ; Liao, Xiao-Lin 1 ; Chen, Song-Lin 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Utilizat Marine Fisheries Res, Qingdao 266071, Peoples R China
2.Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China
关键词: Black snapper;Sebastodes fuscescens;Enriched genomic library;SSR;Microsatellites
期刊名称:CONSERVATION GENETICS ( 影响因子:2.538; 五年影响因子:2.32 )
ISSN: 1566-0621
年卷期: 2009 年 10 卷 5 期
页码:
收录情况: SCI
摘要: Black snapper Sebastodes fuscescens (Houttuyn) is an economocally important species. Fourty-five microsatellite loci were isolated from an enriched genomic library of S. fuscescens. Ten of these loci were polymorphic in a test population with alleles per locus ranging from 2 to 5, and observed and expected heterozygosities per locus from 0.21 to 1.00 and from 0.19 to 0.86, respectively. Three loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of S. fuscescens and other related species.
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