Evaluation of Female Recipient Infertility and Donor Spermatogonial Purification for Germ Cell Transplantation in Paralichthys olivaceus
文献类型： 外文期刊
作者： Ren, Yuqin 1 ; Tao, Yuehong 1 ; Sun, Zhaohui 1 ; Wang, Yufen 1 ; Li, Weidong 4 ; He, Zhongwei 1 ; Wang, Guixing 1 ; Yang, Yucong 1 ; Hou, Jilun 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Hebei Key Lab Bohai Sea Fish Germplasm Resources C, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Peoples R China
2.Chinese Acad Fishery Sci, Bohai Sea Fishery Res Ctr, Qinhuangdao 066100, Peoples R China
3.Shanghai Ocean Univ, Shanghai Collaborat Innovat Aquat Anim Genet & Bre, Shanghai 201306, Peoples R China
4.Tangshan Haidu Aquat Food Co Ltd, Tangshan 063000, Peoples R China
关键词： ovarian depletion; spermatogonia stem cell; transplantation; Paralichthys olivaceus; busulfan
期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）
ISSN： 2076-2615
年卷期： 2024 年 14 卷 19 期
页码：
收录情况： SCI
摘要： Since the advent of germ cell transplantation (GCT), it has been widely used in shortening the fish breeding cycle, sex-controlled breeding and the protection of rare and endangered fish. In this study, the effectiveness of female sterile recipient preparation and donor stem cell isolation and purification were comprehensively evaluated for spermatogonial stem cell transplantation (SSCT) in Paralichthys olivaceus. The best way to prepare sterile recipients was found to be giving three-year-old fish four intraovarian injections of busulfan (20 mg/kg body weight) combined with exposure to a high temperature (28 degrees C) after the spawning season compared with the two other ways, which induced apoptosis of most of the endogenous germ cells, resulting in shrinkage of the spawning plate and enlargement of the ovarian lumen. Further analysis showed that both the gonadosomatic index and germ-cell-specific vasa expression were significantly lower than those of the natural-temperature group before treatment (p < 0.05). A high percentage (>60.00%) of spermatogonial stem cells (SSCs) were obtained after isolation and purification and were transplanted into the prepared recipients. After three weeks of SSCT, the numbers of PKH26-labeled SSCs were increased in the ovaries of the recipients. These findings provide a basis for the establishment of an ideal SSCT technique using P. olivaceus females as the recipients, ultimately contributing to the efficient conservation of male germplasm resources and effective breeding.
- 相关文献
作者其他论文 更多>>
-
A newly developed 20 K SNP array reveals QTLs for disease resistance to Cryptocaryon irritans in tiger pufferfish (Takifugu rubripes)
作者：Li, Yangzhen;Fraslin, Clemence;Chi, Yong;Hu, Yuanri;Wang, Jialin;Chen, Songlin;Li, Yangzhen;Mukiibi, Robert;Robledo, Diego;Li, Yangzhen;Li, Weidong;Li, Weiguo;Hu, Yuanri;Wang, Jialin;Chen, Songlin;Houston, Ross D.;Robledo, Diego
关键词：SNP array; Heritability; Genomic prediction; GWAS; QTL; Selective breeding
-
Identification candidate genes and loci associated with hypermelanosis of the blind side in Japanese flounder ( Paralichthys olivaceus) ) using the combination of RNA-Seq and BSA-Seq
作者：Liu, Mingyang;San, Lize;Zhang, Xiaoyan;Li, Bingbu;Yang, Yucong;Zheng, Xinyu;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Cao, Wei;Ren, Yuqin;Wang, Yufen;Wang, Guixing;Hou, Jilun;Liu, Mingyang;San, Lize;Zhang, Xiaoyan;Li, Bingbu;Yang, Yucong;Zheng, Xinyu;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Cao, Wei;Ren, Yuqin;Wang, Yufen;Wang, Guixing;Hou, Jilun;Liu, Mingyang;San, Lize;Zhang, Xiaoyan;Li, Bingbu;Yang, Yucong;Zheng, Xinyu;Zhang, Yitong;He, Zhongwei;Liu, Yufeng;Cao, Wei;Ren, Yuqin;Wang, Yufen;Wang, Guixing;Hou, Jilun;Zhang, Jian;Fang, Xiao
关键词：Recombinant doubled haploid; Paralichthys olivaceus; BSA-seq; Hypermelanosis; Transcriptomic analysis
-
Genetic diversity study of self-fertilized and gynogenetic Japanese flounder ( Paralichthys olivaceus) in whole genomic level
作者：San, Lize;Wang, Guixing;Zhang, Xiaoyan;Zhang, Yitong;Cao, Wei;He, Zhongwei;Liu, Yufeng;Yang, Yucong;Liu, Mingyang;Wang, Yufen;Hou, Jilun;San, Lize;Wang, Guixing;Zhang, Xiaoyan;Zhang, Yitong;Cao, Wei;He, Zhongwei;Liu, Yufeng;Yang, Yucong;Liu, Mingyang;Ren, Yuqin;Wang, Yufen;Hou, Jilun;San, Lize;Wang, Guixing;Zhang, Xiaoyan;Zhang, Yitong;Cao, Wei;He, Zhongwei;Liu, Yufeng;Yang, Yucong;Liu, Mingyang;Ren, Yuqin;Wang, Yufen;Hou, Jilun
关键词：Self-fertilization; Meiotic gynogenesis; Mitotic gynogenesis; Single nucleotide polymorphism; Genetic diversity
-
First genome-wide association and genomic prediction of ammonia-nitrogen tolerance in tiger pufferfish (Takifugu rubripes)
作者：Chi, Yong;Hu, Yuanri;Chen, Songlin;Li, Yangzhen;Chi, Yong;Chen, Songlin;Li, Yangzhen;Chi, Yong;Li, Weidong;Li, Yangzhen;Mukiibi, Robert;Robledo, Diego;Robledo, Diego
关键词：Fugu; Heritability; Environmental stress; GWAS; QTL; Aquaculture
-
Effect of Temperature on the Early Development of Paralichthys olivaceus Otoliths
作者：Meng, Gao;Tang, Jiabao;Sun, Zhaohui;Si, Fei;Meng, Gao;Tang, Jiabao;Sun, Zhaohui;Si, Fei;Meng, Gao;Sun, Zhaohui;Si, Fei;Tang, Jiabao;Wang, Qinglin;Yu, Shanshan;Wang, Qinglin;Yu, Shanshan
关键词：
Paralichthys olivaceus ; otoliths; development; temperature -
Toxic effects of anionic polyacrylamide on the developmental stages of Oryzias melastigma embryos and larvae
作者：Zhao, Xinya;Ren, Hai;Fu, Huiru;Jin, Xiaomin;Zhao, Xinya;Zan, Jiangwei;Sun, Zhaohui;Xue, Xiangping;Si, Fei;Zhao, Xinya;Zan, Jiangwei;Sun, Zhaohui;Xue, Xiangping;Si, Fei;Zhao, Xinya;Zan, Jiangwei;Sun, Zhaohui;Xue, Xiangping;Si, Fei
关键词：Anionic polyacrylamide; Oryzias melastigma; Oxidative stress; Cardiotoxicity; Developmental toxicity
-
Community Structure and Influencing Factors of Macro-Benthos in Bottom-Seeded Marine Pastures: A Case Study of Caofeidian, China
作者：Xue, Xiangping;Sun, Zhaohui;Zan, Jiangwei;Xu, Xinjing;Liu, Xia;Gao, Song;Si, Fei;Xue, Xiangping;Sun, Zhaohui;Zan, Jiangwei;Xu, Xinjing;Liu, Xia;Gao, Song;Si, Fei;Yun, Long;Wang, Guangyu;Liu, Mingshuai
关键词：marine pastures; benthic organisms; community structure; environmental factors
