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5-HTR1D and 5-HTR2B regulated Cryptocaryon irritans-induced inflammatory response of large yellow croaker Larimichthys crocea

文献类型： 外文期刊

作者： Zhou, Xu 1 ; Li, Jun-jie 1 ; Li, Shuang 1 ; Liu, Hui-hui 1 ; Chi, Chang-feng 1 ;

作者机构： 1.Zhejiang Ocean Univ, Natl & Prov Joint Engn Res Ctr Marine Germplasm Re, Sch Marine Sci & Technol, 1st Haidanan Rd, Zhoushan 316022, Peoples R China

2.Chinese Acad Fishery Sci, Changdao Enhancement & Expt Stn, Yantai 265800, Shandong, Peoples R China

关键词： Larimichthys crocea; 5-hydroxytryptamine receptor; Expression; Cryptocaryon irritans; Inflammatory response

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 319 卷

页码：

收录情况： SCI

摘要： In mammals, 5-hydroxytryptamine receptors (5-HTRs) are expressed on various immune cells, and regulate the host immune response. However, the immunomodulatory function of 5-HTR in fish still remains poorly understood. In this study, Lc_5-HTR1D and Lc_5-HTR2B genes were cloned from large yellow croaker (Larimichthys crocea), showing highly conserved structural characteristics with other vertebrates orthologs. Lc_5-HTR1D and Lc_5-HTR2B mRNAs exhibited high expression not only in the brain, but also in the immune-related tissues (gill and head kidney). Following Cryptocaryon irritans infection, the expressions of Lc_5-HTR1D mRNA were strikingly induced in the brain and immune-related tissues (gill, intestine, liver, head kidney and spleen), whereas Lc_5-HTR2B mRNAs showed notably up-regulation in the liver and head kidney (P < 0.05 or 0.01). Afterwards, 5-HTR1D antagonist (BRL15572) markedly inhibited the expression of TNF-alpha, IL-1 beta, CXCL8, and IL-17A/F3 mRNA in LPS or C. irritans protein-activated head kidney macrophages (HKMs) (P < 0.05). Conversely, 5-HTR2B antagonist (SB204741) notably enhanced TNF-alpha and IL-1 beta mRNA expression in LPS or C. irritans protein-activated HKMs (P < 0.05 or 0.01). Moreover, 5-HTR1D antagonist significantly inhibited the phagocytosis of C. irritans protein-activated HKMs, while 5-HTR2B antagonist distinctly promoted this process (P < 0.05). Finally, 5-HTR1D antagonist notably hindered the phosphorylation levels of JNK and I kappa B-alpha in C. irritans protein-activated HKMs (P < 0.05), whereas 5-HTR2B antagonist exerted no significant effects on p38, JNK and I kappa B-alpha phosphorylation levels in C. irritans protein-activated HKMs. The findings collectively demonstrated the differential and critical roles of Lc_5-HTR1D and Lc_5-HTR2B in regulating the inflammatory response and host defense mechanisms, and provides a new perspective for neuroimmune regulation of teleost.

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