Molecular and Physiological Effects of 17α-methyltestosterone on Sex Differentiation of Black Rockfish, Sebastes schlegelii
文献类型： 外文期刊
作者： Huang, Haijun 1 ; Liu, Yuyan 2 ; Wang, Qian 2 ; Dong, Caichao 2 ; Dong, Le 2 ; Zhang, Jingjing 2 ; Yang, Yu 2 ; Hao, Xiancai 2 ; Li, Weijing 2 ; Rosa, Ivana F. 3 ; Doretto, Lucas B. 2 ; Cao, Xuebin 4 ; Shao, Changwei 2 ;
作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Peoples R China
3.Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, BR-01049010 Botucatu, Brazil
4.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China
5.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
关键词： S. schlegelii; 17 alpha-methyltestosterone; gonadal development; sex reversal; sex hormone; transcriptomics
期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）
ISSN：
年卷期： 2024 年 15 卷 5 期
页码：
收录情况： SCI
摘要： It is widely known that all-female fish production holds economic value for aquaculture. Sebastes schlegelii, a preeminent economic species, exhibits a sex dimorphism, with females surpassing males in growth. In this regard, achieving all-female black rockfish production could significantly enhance breeding profitability. In this study, we utilized the widely used male sex-regulating hormone, 17 alpha-methyltestosterone (MT) at three different concentrations (20, 40, and 60 ppm), to produce pseudomales of S. schlegelii for subsequent all-female offspring breeding. Long-term MT administration severely inhibits the growth of S. schlegelii, while short term had no significant impact. Histological analysis confirmed sex reversal at all MT concentrations; however, both medium and higher MT concentrations impaired testis development. MT also influenced sex steroid hormone levels in pseudomales, suppressing E2 while increasing T and 11-KT levels. In addition, a transcriptome analysis revealed that MT down-regulated ovarian-related genes (cyp19a1a and foxl2) while up-regulating male-related genes (amh) in pseudomales. Furthermore, MT modulated the TGF-beta signaling and steroid hormone biosynthesis pathways, indicating its crucial role in S. schlegelii sex differentiation. Therefore, the current study provides a method for achieving sexual reversal using MT in S. schlegelii and offers an initial insight into the underlying mechanism of sexual reversal in this species.
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