Molecular and Physiological Effects of 17α-methyltestosterone on Sex Differentiation of Black Rockfish, Sebastes schlegelii
文献类型： 外文期刊
作者： Huang, Haijun 1 ; Liu, Yuyan 2 ; Wang, Qian 2 ; Dong, Caichao 2 ; Dong, Le 2 ; Zhang, Jingjing 2 ; Yang, Yu 2 ; Hao, Xiancai 2 ; Li, Weijing 2 ; Rosa, Ivana F. 3 ; Doretto, Lucas B. 2 ; Cao, Xuebin 4 ; Shao, Changwei 2 ;
作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Peoples R China
3.Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, BR-01049010 Botucatu, Brazil
4.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China
5.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
关键词： S. schlegelii; 17 alpha-methyltestosterone; gonadal development; sex reversal; sex hormone; transcriptomics
期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）
ISSN：
年卷期： 2024 年 15 卷 5 期
页码：
收录情况： SCI
摘要： It is widely known that all-female fish production holds economic value for aquaculture. Sebastes schlegelii, a preeminent economic species, exhibits a sex dimorphism, with females surpassing males in growth. In this regard, achieving all-female black rockfish production could significantly enhance breeding profitability. In this study, we utilized the widely used male sex-regulating hormone, 17 alpha-methyltestosterone (MT) at three different concentrations (20, 40, and 60 ppm), to produce pseudomales of S. schlegelii for subsequent all-female offspring breeding. Long-term MT administration severely inhibits the growth of S. schlegelii, while short term had no significant impact. Histological analysis confirmed sex reversal at all MT concentrations; however, both medium and higher MT concentrations impaired testis development. MT also influenced sex steroid hormone levels in pseudomales, suppressing E2 while increasing T and 11-KT levels. In addition, a transcriptome analysis revealed that MT down-regulated ovarian-related genes (cyp19a1a and foxl2) while up-regulating male-related genes (amh) in pseudomales. Furthermore, MT modulated the TGF-beta signaling and steroid hormone biosynthesis pathways, indicating its crucial role in S. schlegelii sex differentiation. Therefore, the current study provides a method for achieving sexual reversal using MT in S. schlegelii and offers an initial insight into the underlying mechanism of sexual reversal in this species.
- 相关文献
作者其他论文 更多>>
-
CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole (Cynoglossus semilaevis) Testis Cells
作者：Sun, Yanxu;Yue, Bowen;Gong, Xiaoling;Sun, Yanxu;Wang, Hong-Yan;Liu, Binghua;Yue, Bowen;Liu, Qian;Liu, Yuyan;Doretto, Lucas B.;Han, Shenglei;Lin, Lei;Shao, Changwei;Rosa, Ivana F.;Gong, Xiaoling;Gong, Xiaoling;Shao, Changwei
关键词：CRISPR/dCas9; DNA methylation; emx2; Cynoglossus semilaevis
-
Molecular functional characterization of the setdb1 and its potential target gene sox5 illuminate the histone modification-mediated orchestration of gonadal development in Chinese tongue sole (Cynoglossus semilaevis)
作者：Yue, Bowen;Yue, Bowen;Wang, Hong-Yan;Huang, Yingyi;Li, Shuo;Ma, Wenxiu;Liu, Qian;Shao, Changwei;Wang, Hong-Yan;Huang, Yingyi;Li, Shuo;Ma, Wenxiu;Liu, Qian;Shao, Changwei;Shao, Changwei
关键词：Setdb1; Gonad; Sox5; Chinese tongue sole
-
Unveiling Gene Expression Dynamics during Early Embryogenesis in Cynoglossus semilaevis: A Transcriptomic Perspective
作者：Cheng, Xinyi;Cheng, Xinyi;Dai, Wei;Li, Qiye;Jiang, Wei;Li, Qiye;Wang, Qian;Liu, Kaiqiang;Liu, Yuyan;Shao, Changwei;Wang, Qian;Liu, Kaiqiang;Liu, Yuyan;Shao, Changwei;Li, Qiye
关键词：Cynoglossus semilaevis; Cynoglossidae; gametes; early embryonic development; transcriptomics
-
Single-cell transcriptome landscape of zebrafish liver reveals hepatocytes and immune cell interactions in understanding nonalcoholic fatty liver disease
作者：Huang, Yingyi;Liu, Xiang;Wang, Hong-Yan;Li, Yubang;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Huang, Yingyi;Liu, Xiang;Wang, Hong-Yan;Li, Yubang;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Chen, Jian-Yang;Zhang, Xianghui;Lu, Yifang;Wang, Qian;Fan, Guangyi;Chen, Jian-Yang;Zhang, Xianghui;Lu, Yifang;Fan, Guangyi;Dong, Zhongdian;Wang, Zhongduo;Liu, Shanshan;Fan, Guangyi;Liu, Shanshan;Zou, Jun
关键词：
-
The Role of Z Chromosome Localization Gene psmd9 in Spermatogenesis of Cynoglossus semilaevis
作者：Zhang, Yuman;Wang, Yue;Liu, Qian;Wang, Hongyan;Wang, Qian;Shao, Changwei;Wang, Qian;Shao, Changwei
关键词：psmd9; spermatogenesis; sex chromosome; overexpression; RNAi
-
Telomere-to-telomere gapless genome assembly of the Chinese sea bass (Lateolabrax maculatus)
作者：Sun, Zhilong;Sun, Zhilong;Li, Shuo;Liu, Yuyan;Li, Weijing;Liu, Kaiqiang;Wang, Hongyan;Wang, Qian;Shao, Changwei;Cao, Xuebin;Lin, Jiliang
关键词：
-
Transcriptome Analysis Revealed the Early Heat Stress Response in the Brain of Chinese Tongue Sole (Cynoglossus semilaevis)
作者：Wang, Yue;Yang, Yanjing;Su, Chengcheng;Liu, Qian;Hao, Xiancai;Han, Shenglei;Doretto, Lucas B.;Shao, Changwei;Wang, Qian;Rosa, Ivana F.;Shao, Changwei;Wang, Qian
关键词：Cynoglossus semilaevis; transcriptome; heat shock; gene
