您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Swine influenza virus triggers ferroptosis in A549 cells to enhance virus replication

文献类型： 外文期刊

作者： Cheng, Jinghua 1 ; Tao, Jie 1 ; Li, Benqiang 1 ; Shi, Ying 1 ; Liu, Huili 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, Shanghai 201106, Peoples R China

2.Shanghai Key Lab Agr Genet Breeding, Shanghai 201106, Peoples R China

3.Shanghai Engn Res Ctr Pig Breeding, Shanghai 201302, Peoples R China

关键词： Swine influenza virus; Proteomics analysis; Ferroptosis; Iron disorder

期刊名称：VIROLOGY JOURNAL （ 影响因子：5.913； 五年影响因子：4.372 ）

ISSN：

年卷期： 2022 年 19 卷 1 期

页码：

收录情况： SCI

摘要： Background: Recently, Influenza A virus (IAV) has been shown to activate several programmed cell death pathways that play essential roles in host defense. Indeed, cell death caused by viral infection may be mediated by a mixed pattern of cell death instead of a certain single mode. Ferroptosis is a novel form of regulated cell death (RCD) that is mainly mediated by iron-dependent lipid peroxidation. Based on the proteomic data, we wondered whether IAV causes ferroptosis in host cells. Method: In this study, a quantitative proteomics approach based on an iTRAQ combined with LC-MS/MS was used to profile proteins expressed in A549 cells infected with H1N1 swine influenza virus (Sly). Meanwhile, we measured the intracellular iron content, reactive oxygen species (ROS) release and lipid peroxidation in response to SIV infection. Finally, a drug experiment was conducted to investigate the effects of ferroptosis on modulating SIV survival. Results: The bioinformatics analysis revealed several proteins closely relevant to iron homeostasis and transport, and the ferroptosis signaling pathway are highly enriched in response to SIV infection. In our experiment, aberrant expression of iron-binding proteins disrupted labile iron uptake and storage after SIV infection. Meanwhile, SIV infection inhibited system the Xc(-)/GPX4 axis resulting in GSH depletion and the accumulation of lipid peroxidation products. Notably, cell death caused by SIV as a result of iron-dependent lipid peroxidation can be partially rescued by ferroptosis inhibitor. Additionally, blockade of the ferroptotic pathway by ferrostatin-1 (Fer-1) treatment decreased viral titers and inflammatory response. Conclusions: This study revealed a new mode of cell death induced by IAV infection, and our findings might improve the understanding of the underlying mechanism involved in the interaction of virus and host cells.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台