您好,欢迎访问中国水产科学研究院 机构知识库!

Development of microsatellite markers and their utilization in genetic diversity analysis of cultivated and wild populations of the mud carp (Cirrhina molitorella)

文献类型: 外文期刊

作者: Yang, Cheng 1 ; Zhu, Xinping 3 ; Sun, Xiaowen 1 ;

作者机构: 1.Chinese Acad Fisheries Sci, Heilongjiang River Fisheries Res Inst, Dept Biotechnol, Harbin 150076, Peoples R China

2.Shanghai Fisheries Univ, Coll Aqualife Sci & Technol, Shanghai 200090, Peoples R China

3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Peoples R China

关键词: microsatellite marker;genetic diversity;mud carp;Cirrhina molitorella

期刊名称:JOURNAL OF GENETICS AND GENOMICS ( 影响因子:4.275; 五年影响因子:5.223 )

ISSN: 1673-8527

年卷期: 2008 年 35 卷 4 期

页码:

收录情况: SCI

摘要: Microsatellite markers have been increasingly used in genetic studies on fishery species because of their high applicability in selective breeding programs. Here we reported the development of microsatellite markers and their utilization in mud carp (Cirrhina molitorella). An (CA)15 enriched library has been constructed for mud carp, using the magnetic beads enrichment procedure. Sequence analysis of 60 randomly picked positive colonies indicate that 56 (93.3%) of the colonies contain microsatellites. Microsatellite polymorphism was assessed using 10 mud carp individuals, and 12 microsatellite loci turned out to be polymorphic. We utilized these loci to study the genetic diversity of a wild population (WM) and a cultured population (CM) of the mud carp. A total of 109 alleles were detected with an average of 9.08 alleles per locus. The mean value of the observed heterozygosity of WM and CM was 0.6361 and 0.6417, respectively, and significant decrease of genetic diversity in CM was not observed. The genetic distance between the two populations was 0.1546 and the value of G(ST) was 0.0473. This showed that there existed a slight genetic differentiation between WM and CM.

  • 相关文献

[1]Genetic diversity analysis of oriental river prawn, Macrobrachium nipponense, in Yellow River using microsatellite marker. Qiao, H.,Jiang, S. F.,Sun, M.,Gong, Y. S.,Xiong, Y. W.,Jin, S. B.,Fu, H. T.,Lv, D.. 2013

[2]Isolation and characterization of polymorphic microsatellite markers from Coilia ectenes. Rong, X. J.,Wang, Q. Y.,Rong, X. J.,Xu, Y. J.,Wang, Q. Y.,Liao, M. J.,Liu, X. Z.,Pan, C. Y.,Zhang, Z.,Wang, Y. G.. 2013

[3]The impacts of invasive Nile tilapia (Oreochromis niloticus) on the fisheries in the main rivers of Guangdong Province, China. Gu, Dang En,Ma, Guang Ming,Zhu, Yun Jie,Xu, Meng,Luo, Du,Li, Ying Ying,Wei, Hui,Mu, Xi Dong,Luo, Jian Ren,Hu, Yin Chang,Gu, Dang En,Xu, Meng,Luo, Du,Wei, Hui,Mu, Xi Dong,Luo, Jian Ren,Hu, Yin Chang,Ma, Guang Ming,Zhu, Yun Jie.

[4]Discovery and characterization of a first set of polymorphic microsatellite markers in Siganus oramin. Liu, Y. -X.,Ma, C. -Y.,Ma, H. -Y.,Feng, C. -L.,Li, S. -J.,Ma, L. -B.,Liu, Y. -X.. 2015

[5]Isolation of novel microsatellite markers from Paralichthys lethostigma (Paralichthyidae) and their cross-species application in Pleuronectiformes. Xu, Y. -J.,Liao, M. -J.,Wang, Y. -G.,Liu, Z. -C.,Qin, P.,Zhang, Z.,Rong, X. -J.,Liu, X. -Z.. 2013

[6]Germplasm of breeding Pseudosciaena crocea as revealed by microsatellite markers. Chang Yumei,Ding Lei,Liang Liqun,Chang Yumei,He Jianguo,Ding Lei,Li Mingyun,Xue Liangyi,Liang Liqun,Lei Qingquan. 2008

[7]Identification of twenty novel polymorphic microsatellite DNA markers from transcripts of the pearl oyster Pinctada fucata using next-generation sequencing approach. Fan, Sigang,Wang, Jingxuan,Huang, Guiju,Liu, Baosuo,Yu, Dahui,Wang, Jingxuan. 2015

[8]Isolation and characterization of novel polymorphic microsatellite markers for Lutjanus erythropterus. Lin, L.,Li, C. H.,Xu, S. N.,Liu, Y.,Xiao, Y. Y.,Lin, L.,Li, C. H.,Xu, S. N.,Liu, Y.,Xiao, Y. Y.. 2015

[9]Genetic variability of half-smooth tongue sole Cynoglossus semilaevis populations using microsatellite markers. Liu Shuaishuai,Ye Naihao,Liu Yunguo,Liu Yunguo,Liu Shuaishuai,Guo Yinghui,Li Junfeng. 2011

[10]Isolation and characterization of microsatellite markers for Glytothorax zanaensis. Liu, Shao-Ping,Yuan, Xi-Ping,Wang, Deng-Qiang,Yue, Xing-Jian,Zhang, Yan,Wang, Ke,Duan, Xin-Bin,Chen, Da-Qing. 2009

[11]Isolation and characterization of twenty microsatellite markers for the endangered fish Alburnus chalcoides. Geng Longwu,Jiang Haifeng,Tong Guangxiang,Lu Cuiyun,Li Chao,Xu Wei. 2013

[12]Isolation and characterization of polymorphic microsatellite markers in the yellow catfish, Pelteobagrus fulvidraco. Hu, Guang-Fu,Liang, Hong-Wei,Li, Zhong,Wang, Chang-Zhong,Wu, Qin-Chao,Liu, Xiang-Jiang,Luo, Xiang-Zhong,Zou, Gui-Wei. 2009

[13]Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho. Liu, Shufang,Liu, Hongbo,Yuan, Yanjiao,Dai, Fangqun,Zhuang, Zhimeng,Liu, Hongbo,Lin, Lin,Yuan, Yanjiao. 2012

[14]Parentage determination of Chinese shrimp (Fenneropenaeus chinensis) based on microsatellite DNA markers. Dong, Shirui,Kong, Jie,Zhang, Tianshi,Meng, Xianhong,Wang, Rucai. 2006

[15]Genomic structure, polymorphism and expression analysis of the growth hormone (GH) gene in female and male Half-smooth tongue sole (Cynoglossus semilaevis). Ma, Qian,Liu, ShuFang,Zhuang, ZhiMeng,Lin, Lin,Sun, ZhongZhi,Liu, ChangLin,Ma, Hui,Tang, QiSheng,Ma, Qian,Lin, Lin,Su, YongQuan,Ma, Hui.

[16]Induction of meiotic gynogenesis in paddlefish (Polyodon spathula) and its confirmation using microsatellite markers. Zou, Y. C.,Zou, Y. C.,Wei, Q. W.,Pan, G. B.,Wei, Q. W.,Pan, G. B..

[17]Development and characterization of twenty microsatellite markers for the endangered fish Luciobarbus capito. Geng Longwu,Lu Cuiyun,Tong Guangxiang,Li Chao,Xu Wei.

[18]Taxonomic status and genetic diversity of cultured largemouth bass Micropterus salmoides in China. Bai, Junjie,Quan, Yingchun,Liang, Suxian,Lutz-Carrillo, Dijar J.,Liang, Suxian.

[19]Genetic diversity in three Japanese flounder (Paralichthys olivaceus) populations revealed by ISSR markers. Liu, YG,Chen, SL,Li, J,Li, BF. 2006

[20]Genetic structure and low-genetic diversity suggesting the necessity for conservation of the Chinese Longsnout catfish, Leiocassis longirostris (Pisces : Bagriidae). Wang, ZW,Zhou, JF,Ye, YZ,Wei, QW,Wu, QJ. 2006

作者其他论文 更多>>