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Functional Characterization of a Candidate Sex Pheromone Receptor AlinOR33 Involved in the Chemoreception of Adelphocoris lineolatus

文献类型： 外文期刊

作者： Wang, Qi 1 ; Xiao, Yong 1 ; An, Xing-Kui 1 ; Shan, Shuang 1 ; Khashaveh, Adel 1 ; Gu, Shao-Hua 3 ; Zhang, Yun-Hui 1 ; Zha 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

2.Guangdong Acad Agr Sci, Inst Plant Protect, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China

3.China Agr Univ, Coll Plant Protect, Beijing 100193, Peoples R China

关键词： Adelphocoris lineolatus; sex pheromone; odorant receptor; two-electrode voltage-clamp recording; RNA interference; electrophysiological response; behavior change

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2021 年 69 卷 24 期

页码：

收录情况： SCI

摘要： Sex pheromones are deemed to play a significant role in sexual communication of most insects. Although many sex pheromone components in mind bugs have been identified, the roles of odorant receptors in sex pheromone perception in Adelphocoris spp. (Hemiptera: Miridae) remain unknown so far. Here, AlinOR33, a candidate sex pheromone receptor in Adelphocoris lineolatus was functionally characterized. Phylogenetic analysis showed that AlinOR33 clustered with the sex pheromone receptor AlucOR4 fromApolygus lucorum. Quantitative real-time PCR measurement revealed that the expression of AlinOR33 increased gradually from nymph to adult stage and reached its peak in the antennae of 3-day-old mated male bugs. The subsequent in situ hybridization demonstrated that AlinOR33 was mainly expressed in sensilla trichoid on the antennae of A. lineolatus. In the two-electrode voltage clamp recordings, AlinOR33/AlinOrco was specifically tuned to four sex pheromone components including butyl butyrate, hexyl hexanoate, trans-2-hexenyl butyrate and hexyl butyrate, and especially most sensitive to the major component trans-2-hexenyl butyrate. After dsAlinOR33 injection, the electroantennogram responses of males to four sex pheromone components were reduced significantly (similar to 50%). Compared to control bugs, dsAlinOR33-injected male bugs almost lost behavioral preference for trans-2-hexenyl butyrate. Furthermore, the wingbeat frequency of dsAlinOR33-injected male bugs notably declined. Therefore, we conclude that as a candidate sex pheromone receptor, AIinOR33 plays essential roles in the sexual behavior of A. lineolatus.

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