Establishment and transcriptomic analyses of a cattle rumen epithelial primary cells (REPC) culture by bulk and single-cell RNA sequencing to elucidate interactions of butyrate and rumen development
文献类型： 外文期刊
作者： Lin, Shudai 1 ; Fang, Lingzhao 1 ; Kang, Xiaolong 1 ; Liu, Shuli 1 ; Liu, Mei 1 ; Connor, Erin E. 1 ; Baldwin, Ransom L. 1 ; Liu, George 1 ; Li, Cong-Jun 1 ;
作者机构： 1.ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA
2.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Minist Agr, Coll Anim Sci, Guangzhou 510642, Peoples R China
3.South China Agr Univ, Key Lab Chicken Genet Breeding & Reprod, Minist Agr, Coll Anim Sci, Guangzhou 510642, Peoples R China
4.Univ Edinburgh, MRC, Human Genet Unit, Inst Genet & Mol Med, Edinburgh EH4 2XU, Midlothian, Scotland
5.Ningxia Univ, Coll Agr, Yinchuan 750021, Ningxia, Peoples R China
6.China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China
7.Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Agr Mol Biol, Yangling 712100, Shaanxi, Peoples R China
关键词： Agriculture; Bioinformatics; Cell biology; Developmental biology; Butyrate; Epigenetic regulation; Epithelium; Rumen; Transcriptome
期刊名称：HELIYON （ 影响因子：3.776； 五年影响因子：3.752 ）
ISSN：
年卷期： 2020 年 6 卷 6 期
页码：
收录情况： SCI
摘要： As a critical and high -value tool to study the development of rumen, we established a stable rumen epithelial primary cell (REPC) culture from a two-week-old Holstein bull calf rumen epithelial tissue The transcriptomic profiling of the REPC and the direct effects of butyrate on gene expression were assessed. Correlated gene networks elucidated the putative roles and mechanisms of butyrate action in rumen epithelial development. The top networks perturbed by butyrate were associated with epithelial tissue development. Additionally, two critical upstream regulators, E2F1 and TGFB1, were identified to play critical roles in the differentiation, development, and growth of epithelial cells. Significant expression changes of upstream regulators and transcription factors provided further evidence in support that butyrate plays a specific and central role in regulating genomic and epigenomic activities influencing rumen development. This work is the essential component to obtain a complete global landscape of regulatory elements in cattle and to explore the dynamics of chromatin states in rumen epithelial cells induced by butyrate at early developmental stages.
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