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Molecular identification and recombinant expression of red sea bream (Chrysophrys major) hepcidin gene

文献类型： 外文期刊

作者： Chen, Song-Lin 1 ; Sui, Shao-Fei 1 ; Xu, Mei-Yu 1 ; Liu, Yang 1 ; Yang, Rong 1 ;

作者机构： 1.Chinese Acad Fisheries Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Yellow Sea Fisheries Res Inst, Minist Agr, Qingdao 266071, Peoples R China

关键词： antimicrobial peptides;hepcidin;recombinant expression, antibacterial activity;Pichia pastoris;red sea bream;Chrysophrys major

期刊名称：Proceedings of the 13th International Congress on Genes, Gene Families and Isozymes

ISSN：

年卷期： 2005 年

页码：

收录情况： SCI

摘要： Hepcidin is a member of antimicrobial peptides (AMPs) family. However, little is known about the structure and function of hepcidin in lower vertebrates such as fish. We have recently cloned a cDNA encoding hepcidin from cDNA library of spleen of red sea bream (Chrysophrys major) by EST analysis (Chen et al., 2005). In order to study the function of hepcidin in fish, an expression cassette harboring red sea bream hepcidin cDNA was constructed by using secretory plasmid pPICZaA and recombinant hepcidin was generated in yeast (Pichia pastoris). SDS-PAGE of the yeast cultures demonstrated that recombinant hepcidins were produced in the recombinant yeast. The supernatant from recombinant yeast culture exhibited a dose-dependant antibacterial activity on the growth of E. coli DH5a, E. coli ATCC25922, Pseudomonas aeruginosa and Aeromonas hydrophia.

  • 相关文献

[1]Isolation and characterization of polymorphic microsatellite loci from an EST-library of red sea bream (Chrysophrys major) and cross-species amplification. Chen, SL,Liu, YG,Xu, MY,Li, J. 2005

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