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Recombinase polymerase amplification assay for rapid detection of Seneca Valley Virus

文献类型： 外文期刊

作者： Wang, Hua 1 ; Ding, Xinli 2 ; Sun, Wenbo 3 ; Chen, Zhi 3 ; Bai, Linyi 1 ; Liang, Hongkun 1 ; Liu, Yujie 1 ; Zhang, Wanli 4 ; Wang, Guisheng 5 ; Yang, Guiwen 1 ; Mauk, Michael G. 6 ; Cui, Yuanyuan 1 ; Chen, Lei 1 ;

作者机构： 1.Shandong Normal Univ, Coll Life Sci, Shandong Prov Key Lab Anim Resistance Biol, 88 East Wenhua Rd, Jinan 250014, Peoples R China

2.Shandong Inst Commerce & Technol, Dept Food & Drug, 4516 Lvyou Rd, Jinan 250103, Peoples R China

3.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Shandong Key Lab Anim Dis Control & Breeding, Jinan 250100, Peoples R China

4.Shandong Univ TCM, Chinese Massage Dept, Affiliated Hosp, 16369 Jingshi Rd, Jinan 250014, Peoples R China

5.Shandong Prov Ctr Anim Dis Control & Prevent, 4566 Tangye West Rd, Jinan 250010, Shandong, Peoples R China

6.Univ Penn, Sch Engn & Appl Sci, Dept Mech Engn, Philadelphia, PA USA

关键词： Seneca valley virus; Primers and probes; Recombinase polymerase amplification

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.191； 五年影响因子：3.228 ）

ISSN： 0003-2697

年卷期： 2022 年 642 卷

页码：

收录情况： SCI

摘要： Seneca Valley virus (SVV) is related to vesicular disease in pigs, and its clinical symptoms are indistinguishable from other notifiable clinical symptoms of vesicular disease such as foot-and-mouth disease. The rapid and ac-curate detection of SVV is essential to confirm the pathogenic factors and initiate the implementation of control measures. The development of a rapid, simple, convenient, and low-cost molecular (nucleic acid amplification) test that can be used at the sample collection point has been identified as a key component for controlling SVV. This study describes the development and demonstration of recombinase polymerase amplification (RPA) test targeting the conserved regions of SVV for detection of SVV. The Primers and probes designed by us have shown good sensitivity and specificity in RPA test, which is helpful for RPA to be an effective tool for rapid diagnosis of SVV.

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