文献类型： 外文期刊

作者： Li, Yi-Han ¹ ; Peng, Jing ¹ ; Wu, Qing-Jun ³ ; Sun, Jing-Chen ⁴ ; Zhang, Peng-Jun ⁵ ; Qiu, Bao-Li ² ;

作者机构： 1.South China Agr Univ, Engn Res Ctr Biocontrol, Minist Educ & Guangdong Prov, Guangzhou 510640, Peoples R China

2.Chongqing Normal Univ, Engn Res Ctr Biotechnol Act Subst, Minist Educ, Chongqing 401331, Peoples R China

3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

4.South China Agr Univ, Coll Anim Sci, Guangdong Prov Key Lab Agroanim Genom & Mol Breedi, Guangzhou 510642, Guangdong, Peoples R China

5.Hangzhou Normal Univ, Coll Life & Environm Sci, Dept Biol Sci, Hangzhou 311121, Peoples R China

关键词： whitefly; Rickettsia; interaction; transcriptome

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.2； 五年影响因子：2.3 ）

ISSN： 0022-0493

年卷期： 2024 年 117 卷 3 期

页码：

收录情况： SCI

摘要： Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is a destructive insect pest of many crops. Rickettsia infection in different cryptic species of B. tabaci has been observed worldwide. Understanding the interactions between these 2 organisms is critical to developing Rickettsia-based strategies to control B. tabaci and thereby reduce the transmission of related vector-borne viruses. In this study, we investigated the effects of Rickettsia infection on the biological characteristics of the Middle East Asia Minor 1 (MEAM1) strain of B. tabaci through biological analysis of infected and uninfected individuals. The results of this study suggest that Rickettsia may confer fitness benefits. These benefits include increased fertility, improved survival rates, accelerated development, and resulted in female bias. We also investigated the transcriptomics impact of Rickettsia infection on B. tabaci by performing a comparative RNA-seq analysis of nymphs and adult females, both with and without the infection. Our analysis revealed 218 significant differentially expressed genes (DEGs) in infected nymphs compared to uninfected ones and 748 significant DEGs in infected female adults compared to their uninfected whiteflies. Pathway analysis further revealed that Rickettsia can affect many important metabolic pathways in whiteflies. The results suggest that Rickettsia plays an essential role in energy metabolism, and nutrient synthesis in the B. tabaci MEAM1, and depends on metabolites obtained from the host to ensure its survival. Overall, our findings suggest that Rickettsia has beneficial effects on B. tabaci and offered insights into the potential molecular mechanisms governing the interactions between Rickettsia and B. tabaci MEAM1.