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Digital gene expression profiling in larvae of Tribolium castaneum at different periods post UV-B exposure

文献类型： 外文期刊

作者： Guo, Shu-Hao 1 ; Yu, Lin 2 ; Liu, Yan-Mei 1 ; Wang, Fei-Feng 1 ; Chen, Yu-Chen 1 ; Wang, Ye 1 ; Qiu, Bao-Li 1 ; Sang, Wen 1 ;

作者机构： 1.South China Agr Univ, Key Lab Biopesticide Creat & Applicat, Guangzhou 510640, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Guangdong, Peoples R China

关键词： UV-B; Tribolium castaneum; Digital gene expression; Physiological response

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.291； 五年影响因子：6.393 ）

ISSN： 0147-6513

年卷期： 2019 年 174 卷

页码：

收录情况： SCI

摘要： UV-B radiation is an important environmental factor. Exposure to excess UV-B radiation can cause serious effects on the development, survival, and reproduction of different organisms. Plants and animals have developed many different strategies to cope with UV-B-induced damage, but the physiological response of insects to UV-B remains unclear. In the present study, the red flour beetle Tribolium castaneum (Herbst) was used to assess the stress response of UV-B. The underlying molecular mechanisms were explored using RNA sequencing. We investigated the transcriptomic profile of T. castaneum larvae at 4 and 24 h after treatment with UV-B radiation via digital gene expression analysis. The 310 and 996 differentially expressed genes were detected at 4 and 24 h, respectively. Then the biological functions and associated metabolic processes of these genes were determined by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis. The reliability of the data was verified using qRT-PCR. The results indicated that several differentially expressed genes are involved in anti oxidation, DNA repair, protein folding, carbon flux diversion, and the extracellular matrix to protect against UV-B -induced damage. This study will increase our understanding of the molecular mechanism underlying insect response to UV-B radiation.

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