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Coinfection with PEDV and BVDV induces inflammatory bowel disease pathway highly enriched in PK-15 cells

文献类型： 外文期刊

作者： Cheng, Jinghua 1 ; Tao, Jie 1 ; Li, Benqiang 1 ; Shi, Ying 1 ; Liu, Huili 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, 2901 Beidi Rd, Shanghai 201106, Peoples R China

2.Shanghai Key Lab Agr Genet Breeding, Shanghai 201106, Peoples R China

3.Shanghai Engn Res Ctr Pig Breeding, Shanghai 201302, Peoples R China

关键词： Porcine epidemic diarrhea virus; Bovine viral diarrhea virus; Coinfection; Proteomics; Pathway analysis

期刊名称：VIROLOGY JOURNAL （ 影响因子：5.913； 五年影响因子：4.372 ）

ISSN：

年卷期： 2022 年 19 卷 1 期

页码：

收录情况： SCI

摘要： Background From the 1078 diarrhea stools tested in our survey from 2017 to 2020 in local area of China, PEDV was the key pathogen that was closely related to the death of piglets with diarrhea. In addition, coinfection of PEDV-positive samples with BVDV reached 17.24%. Although BVDV infection in swine is typically subclinical, the effect of PEDV and BVDV coinfection on disease severity and the potential molecular mechanism of coinfection with these two viruses remain unknown. Methods In this study, we developed a model of coinfection with porcine epidemic diarrhea virus (PEDV) and bovine viral diarrhea virus (BVDV) in PK15 cells, and a tandem mass tag (TMT) combined with LC-MS/MS proteomic approach was used to identify differential protein expression profiles. Additionally, we performed drug experiments to explore the inflammatory response induced by PEDV or BVDV mono- or coinfection. Results A total of 1094, 1538, and 1482 differentially expressed proteins (DEPs) were identified upon PEDV monoinfection, BVDV monoinfection and PEDV/BVDV coinfection, respectively. KEGG pathway analysis revealed that PEDV and BVDV coinfection led to a highly significantly enrichment of the inflammatory bowel disease (IBD) pathway. In addition, the NF-kappa B signaling pathway was more intensively activated by PEDV and BVDV coinfection, which induced higher production of inflammatory cytokines, than PEDV or BVDV monoinfection. Conclusions Our study indicated that cattle pathogens might play synergistic roles in the pathogenesis of porcine diarrhea, which might also improve our understanding of the pathogenesis of multiple infections in diarrhea.

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