您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Thyroid Hormone Signaling Is Required for Dynamic Variation in Opsins in the Retina during Metamorphosis of the Japanese Flounder (Paralichthys olivaceus)

文献类型： 外文期刊

作者： Shi, Yaxin 1 ; Shi, Yang 4 ; Ji, Wenyao 1 ; Li, Xike 1 ; Shi, Zhiyi 1 ; Hou, Jilun 5 ; Li, Wenjuan 1 ; Fu, Yuanshuai 1 ;

作者机构： 1.Shanghai Ocean Univ, Key Lab Freshwater Aquat Genet Resources, Minist Agr, Shanghai 201306, Peoples R China

2.Shanghai Ocean Univ, Key Lab Explorat & Utilizat Aquat Genet Resources, Minist Educ, Shanghai 201306, Peoples R China

3.Shanghai Ocean Univ, Shanghai Collaborat Innovat Aquat Anim Genet & Bre, Shanghai 201306, Peoples R China

4.Shanghai Food Res Inst, Shanghai 200235, Peoples R China

5.Chinese Acad Fishery Sci, Hebei Key Lab Bohai Sea Fish Germplasm Resources C, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Peoples R China

关键词： Paralichthys olivaceus; opsin; thyroid hormone; metamorphosis

期刊名称：BIOLOGY-BASEL （ 影响因子：4.2； 五年影响因子：4.4 ）

ISSN：

年卷期： 2023 年 12 卷 3 期

页码：

收录情况： SCI

摘要： Simple Summary Fish adapt to changes in their external light environment by adjusting their visual system. Fish that live at different depths can perceive different spectral ranges, leading to differences in the type and expression of their optic proteins. The Japanese flounder (Paralichthys olivaceus) is a valuable cold temperate benthic marine fish that undergoes a series of metamorphoses during its juvenile stage. The vision of flounder larvae is reshaped to adapt to changes in their light environment. Previous studies have shown that thyroid hormone (TH) signaling is critical for flounder metamorphosis. In the present study, we investigated the tissue composition and the metamorphosis temporal expression profile of five opsin genes in flounder larvae. We also investigated the effect of TH on the opsin genes' expression levels by adding TH and thiourea (a TH inhibitor, TU) to the culture water. In addition, we investigated the thyroid hormone receptor (TR)-mediated targeting regulatory relationship between TH and the opsin genes to further explore the function of TH in visual remodeling during flounder metamorphosis. In the present study, we investigated the function of thyroid hormones (TH) in visual remodeling during Japanese flounder (Paralichthys olivaceus) metamorphosis through cellular molecular biology experiments. Our results showed that the expression of the five opsin genes of the flounder were highest in eye tissue and varied with the metamorphosis process. The expression of rh1, sws2a beta and lws was positively regulated by exogenous TH, but inhibited by thiourea (TU) compared to the control group. In addition, there was a significant increase in sws2a beta and lws in the rescue experiments performed with TU-treated larvae (p < 0.05). Meanwhile, T3 levels in flounder larvae were increased by TH and decreased by TU. Based on the differences in the expression of the three isoforms of the thyroid hormone receptor (TR) (Tr alpha a, Tr alpha b and Tr beta), we further hypothesized that T3 may directly or indirectly regulate the expression of sws2a beta through Tr alpha a. This study demonstrates the regulatory role of TH in opsins during flounder metamorphosis and provides a basis for further investigation on the molecular mechanisms underlying the development of the retinal photoreceptor system in flounders.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台