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SSR marker based analysis for identification and of genetic diversity of non-heading Chinese cabbage varieties

文献类型： 外文期刊

作者： Yin, Jiwei 1 ; Zhao, Hong 2 ; Wu, Xingting 3 ; Ma, Yingxue 3 ; Zhang, Jingli 2 ; Li, Ying 4 ; Shao, Guirong 5 ; Chen, Hairong 2 ; Han, Ruixi 3 ; Xu, Zhenjiang 1 ;

作者机构： 1.South China Agr Univ, Coll Agr, Guangzhou, Peoples R China

2.Shanghai Acad Agr Sci, Inst Agri Food Stand & Testing Technol, Shanghai, Peoples R China

3.Minist Agr & Rural Affairs, Dev Ctr Sci & Technol, Beijing, Peoples R China

4.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Peoples R China

5.Fujian Jinpin Agr Technol Co Ltd, Res & Dev Ctr, Fuzhou, Peoples R China

关键词： non-heading Chinese cabbage; SSR; variety identification; DUS test; genetic diversity

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： As a widely cultivated vegetable in China and Southeast Asia, the breeding of non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) is widespread; more than 400 varieties have been granted new plant variety rights (PVRs) in China. Distinctness is one of the key requirements for the granting of PVRs, and molecular markers are widely used as a robust supplementary method for similar variety selection in the distinctness test. Although many genome-wide molecular markers have been developed, they have not all been well used in variety identification and tests of distinctness of non-heading Chinese cabbage. In this study, by using 423 non-heading Chinese cabbage varieties collected from different regions of China, 287 simple sequence repeat (SSR) markers were screened for polymorphisms, and 23 core markers were finally selected. The polymorphic information content (PIC) values of the 23 SSR markers ranged from 0.555 to 0.911, with an average of 0.693, and the average number of alleles per marker was 13.65. Using these 23 SSR markers, 418 out of 423 varieties could be distinguished, with a discrimination rate of 99.994%. Field tests indicated that those undistinguished varieties were very similar and could be further distinguished by a few morphological characteristics. According to the clustering results, the 423 varieties could be divided into three groups: pak-choi, caitai, and tacai. The similarity coefficient between the SSR markers and morphological characteristics was moderate (0.53), and the efficiency of variety identification was significantly improved by using a combination of SSR markers and morphological characteristics.

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