ZmG6PDH1 in glucose-6-phosphate dehydrogenase family enhances cold stress tolerance in maize
文献类型： 外文期刊
作者： Li, Xin 1 ; Cai, Quan 2 ; Yu, Tao 2 ; Li, Shujun 2 ; Li, Sinan 2 ; Li, Yunlong 2 ; Sun, Yan 2 ; Ren, Honglei 2 ; Zhang, Jiajia 3 ; Zhao, Ying 3 ; Zhang, Jianguo 2 ; Zuo, Yuhu 1 ;
作者机构： 1.Heilongjiang Bayi Agr Univ, Natl Coarse Cereals Engn Res Ctr, Heilongjiang Prov Key Lab Crop Pest Interact Biol, Daqing, Heilongjiang, Peoples R China
2.Heilongjiang Acad Agr Sci, Key Lab Maize Genet & Breeding, Harbin, Heilongjiang, Peoples R China
3.Northeast Agr Univ, Coll Agr, Harbin, Heilongjiang, Peoples R China
关键词： ZmG6PDH1; enzyme activity; expression; cold stress; CRISPR; Cas9; maize (Zea mays L; )
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2023 年 14 卷
页码：
收录情况： SCI
摘要： Glucose-6-phosphate dehydrogenase (G6PDH) is a key enzyme in the pentose phosphate pathway responsible for the generation of nicotinamide adenine dinucleotide phosphate (NADPH), thereby playing a central role in facilitating cellular responses to stress and maintaining redox homeostasis. This study aimed to characterize five G6PDH gene family members in maize. The classification of these ZmG6PDHs into plastidic and cytosolic isoforms was enabled by phylogenetic and transit peptide predictive analyses and confirmed by subcellular localization imaging analyses using maize mesophyll protoplasts. These ZmG6PDH genes exhibited distinctive expression patterns across tissues and developmental stages. Exposure to stressors, including cold, osmotic stress, salinity, and alkaline conditions, also significantly affected the expression and activity of the ZmG6PDHs, with particularly high expression of a cytosolic isoform (ZmG6PDH1) in response to cold stress and closely correlated with G6PDH enzymatic activity, suggesting that it may play a central role in shaping responses to cold conditions. CRISPR/Cas9-mediated knockout of ZmG6PDH1 on the B73 background led to enhanced cold stress sensitivity. Significant changes in the redox status of the NADPH, ascorbic acid (ASA), and glutathione (GSH) pools were observed after exposure of the zmg6pdh1 mutants to cold stress, with this disrupted redox balance contributing to increased production of reactive oxygen species and resultant cellular damage and death. Overall, these results highlight the importance of cytosolic ZmG6PDH1 in supporting maize resistance to cold stress, at least in part by producing NADPH that can be used by the ASA-GSH cycle to mitigate cold-induced oxidative damage.
- 相关文献
作者其他论文 更多>>
-
Genome-Wide Identification and Expression Analysis Under Abiotic Stress of the Lipoxygenase Gene Family in Maize (Zea mays)
作者：Li, Sinan;Hou, Shuai;Sun, Yuanqing;Sun, Minghao;Sun, Yan;Li, Xin;Li, Yunlong;Wang, Luyao;Cai, Quan;Guo, Baitao;Zhang, Jianguo
关键词：maize; LOX; abiotic stress; gene family; expression analysis
-
Comparative Metabolomics Analysis of Seed Composition Accumulation in Soybean (Glycine max L.) Differing in Protein and Oil Content
作者：Cui, Yifan;Wang, Zhiyang;Li, Mingyang;Wang, Sihui;Liu, Chunyan;Xin, Dawei;Qi, Zhaoming;Chen, Qingshan;Yang, Mingliang;Zhao, Ying;Li, Xin
关键词：metabolomics; seed oil; seed protein; soybean (Glycine max (Linn.) Merr.)
-
Characterization of the soybean ABF gene family and the key regulatory function of GmABF1 in salt stress response
作者：Zhang, Bin-shuo;Li, Ming-yang;Han, Jing-pei;Cheng, Zi-qian;Hu, Zhen-bang;Liu, Chun-yan;Yang, Ming-liang;Wu, Xiao-xia;Zhao, Ying;Song, Zhen;Chen, Xiang-jin;Han, De-zhi;Wu, Xiao-xia;Qiu, Li-juan
关键词：Soybean; ABF; Abiotic stress; Transgenic plants
-
Synergistic effects of foliar applied glycine betaine and proline in enhancing rice yield and stress resilience under salinity conditions
作者：Lamlom, Sobhi F.;Lamlom, Sobhi F.;El-Banna, Aly A. A.;El-Sorady, Gawhara A.;Ren, Honglei;El-Yamany, Bassant A. M.;Salama, Ehab A. A.;Kamara, Mohamed M.;AlGarawi, Amal Mohamed;Hatamleh, Ashraf Atef;Shehab, Abdelsalam A.;Abdelghany, Ahmed M.
关键词：Rice (Oryza sativa L.); Salt stress; Path analysis; Clustering heatmap; Climate change
-
Comprehensively characterize the soybean CAM/CML gene family, as it provides resistance against both the soybean mosaic virus and Cercospora sojina pathogens
作者：Zhang, Chunlei;Wang, Yanbo;Zhang, Ruiping;Yuan, Rongqiang;Zhao, Kezhen;Liu, Xiulin;Wang, Xueyang;Zhang, Fengyi;Lamlom, Sobhi F.;Ren, Honglei;Wang, Yanbo;Lamlom, Sobhi F.;Zhang, Bixian
关键词：soybean mosaic virus (SMV);
C. sojina ; soybean; CAM/CML genes; disease resistance -
Genome-Wide Association Study Reveals Key Genetic Loci Controlling Oil Content in Soybean Seeds
作者：Wang, Xueyang;Zhang, Min;Liu, Xiulin;Zhang, Chunlei;Zhang, Fengyi;Zhao, Kezhen;Yuan, Rongqiang;Lamlom, Sobhi F.;Ren, Honglei;Zhang, Min;Li, Fuxin;Qiu, Hongmei;Lamlom, Sobhi F.;Zhang, Bixian
关键词：soybean; oil content; genome-wide association study (GWAS); single-nucleotide polymorphism (SNP); Northern China
-
Biochemical and yield response of spring wheat to drought stress through gibberellic and abscisic acids
作者：Lamlom, Sobhi F.;El-Sorady, Gawhara A.;Abdelghany, Ahmed M.;Farouk, A. S.;Alwakel, E. Sh.;Makled, Khaled M.;Shehab, A. A.;Bukhari, Najat A.;Hatamleh, Ashraf Atef;Ren, Honglei
关键词：Antioxidant activities; Drought stress; Plant growth regulators; Reactive oxygen species (ROS)
