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Open Fermentative Production of L-Lactic Acid from Distillers' Grains by Lactobacillus casei CICC 6056

文献类型： 外文期刊

作者： Zheng, Jin 1 ; Liu, Yingying 2 ; Sun, Xiaohong 3 ; Wang, Qunhui 1 ; Zou, Hui 1 ; Wang, Juan 1 ; Gao, Ming 4 ;

作者机构： 1.Univ Sci & Technol Beijing, Sch Civil & Environm Engn, Dept Environm Engn, 30 Xueyuan Rd, Beijing 100083, Peoples R China

2.China Natl Inst Standardizat, 4 Zhi Chun Rd, Beijing 100088, Peoples R China

3.Beijing Acad Agr & Forestry Sci, 11 Shuguang Garden Rd, Beijing 100097, Peoples R China

4.Kyushu Univ, Lab Microbial Technol, Div Syst Bioengn, Dept Biosci & Biotechnol,Fac Agr,Grad Sch,Higashi, 6-10-1 Hakozaki, Fukuoka 8128581, Japan

关键词： Open fermentation;L-Lactic production;Distillers'grains;Lactobacillus casei

期刊名称：BIORESOURCES （ 影响因子：1.614； 五年影响因子：1.923 ）

ISSN： 1930-2126

年卷期： 2017 年 12 卷 1 期

页码：

收录情况： SCI

摘要： Distillers' grains (DG) are potential fermentable sugar substrates for the production of value-added products. This study focused on the development of an open fermentation process for the production of L-lactic acid from DG using Lactobacillus casei CICC 6056. The open fermentation process was feasible, resulting in an L-lactic acid yield similar to that obtained with sterilized fermentation. However, a decrease in pH below 5.0 after 24 h resulted in a poor L-lactic acid concentration of 9.18 g/L and an inferior reducing sugar conversion rate of 53.6%. Therefore, an optimal pH adjustment method was sought. A pH adjustment to 6.5 every 24 h effectively improved the L-lactic acid concentration to 21.3 g/L with a 97.8% reducing sugar conversion rate. Furthermore, the application of a simultaneous saccharification and fermentation process at 50 degrees C in open condition increased the L-lactic acid concentration to 25.0 g/L. This yield was superior to L-lactic acid fermentation from DG using a separate hydrolysis and fermentation method (21.3 g/L) and from a commercial sugar in batch culture (23.6 g/L). These results demonstrated that L. casei CICC 6056 has great industrial potential as a superior candidate strain for the production of L-lactic acid from DG due to its broad applicability in a wide temperature range (from 37 to 50 degrees C).

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