Comparative Profiling of microRNA Expression in Soybean Seeds from Genetically Modified Plants and their Near-Isogenic Parental Lines
文献类型： 外文期刊
作者： Wang, Yong 1 ; Lan, Qingkuo 2 ; Zhao, Xin 2 ; Xu, Wentao 3 ; Li, Feiwu 4 ; Wang, Qinying 1 ; Chen, Rui 2 ;
作者机构： 1.Agr Univ Hebei, Coll Plant Protect, Baoding 071001, Peoples R China
2.Tianjin Acad Agr Sci, Tianjin Inst Agr Qual Stand & Testing Technol, Tianjin 300381, Peoples R China
3.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China
4.Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun 130033, Peoples R China
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2016 年 11 卷 5 期
页码：
收录情况： SCI
摘要： MicroRNAs (miRNAs) have been widely demonstrated to play fundamental roles in gene regulation in most eukaryotes. To date, there has been no study describing the miRNA composition in genetically modified organisms (GMOs). In this study, small RNAs from dry seeds of two GM soybean lines and their parental cultivars were investigated using deep sequencing technology and bioinformatic approaches. As a result, several differentially expressed gma-miRNAs were found between the GM and non-GM soybeans. Meanwhile, more differentially expressed gma-miRNAs were identified between distantly relatednon-GM soybeans, indicating that the miRNA components of soybean seeds varied among different soybean lines, including the GM and non-GM soybeans, and the extent of difference might be related to their genetic relationship. Additionally, fourteen novel gma-miRNA candidates were predicted in soybean seeds including a potential bidirectionally transcribed miRNA family with two genomic loci (gma-miR-N1). Our findings firstly provided useful data for miRNA composition in edible GM crops and also provided valuable information for soybean miRNA research.
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