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Isolation and Characterization of a Dominant Dwarf Gene, D-h, in Rice

文献类型： 外文期刊

作者： Piao, Rihua 1 ; Chu, Sang-Ho 1 ; Jiang, Wenzhu 3 ; Yu, Yoye 1 ; Jin, Yongmei; Woo, Mi-Ok 1 ; Lee, Joohyun; Kim, Sun 1 ;

作者机构： 1.Seoul Natl Univ, Res Inst Agr & Life Sci, Dept Plant Sci, Seoul, South Korea

2.Seoul Natl Univ, Coll Agr & Life Sci, Plant Genom & Breeding Inst, Seoul, South Korea

3.Jilin Univ, Coll Plant Sci, Changc

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2014 年 9 卷 2 期

页码：

收录情况： SCI

摘要： Plant height is an important agronomic trait that affects grain yield. Previously, we reported a novel semi-dominant dwarfmutant, HD1, derived from chemical mutagenesis using N-methyl-N-nitrosourea (MNU) on a japonica rice cultivar, Hwacheong. In this study, we cloned the gene responsible for the dwarf mutant using a map-based approach. Fine mapping revealed that the mutant gene was located on the short arm of chromosome 1 in a 48 kb region. Sequencing of the candidate genes and rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) analysis identified the gene, d-h, which encodes a protein of unknown function but whose sequence is conserved in other cereal crops. Real-time (RT)-PCR analysis and promoter activity assays showed that the d-h gene was primarily expressed in the nodes and the panicle. In the HD1 plant, the d-h gene was found to carry a 63-bp deletion in the ORF region that was subsequently confirmed by transgenic experiments to be directly responsible for the gain-of-function phenotype observed in the mutant. Since the mutant plants exhibit a defect in GA response, but not in the GA synthetic pathway, it appears that the d-h gene may be involved in a GA signaling pathway.

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