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Cloning and polymorphisms of the 3 ' untranslated region of malic enzyme gene in Chinese red cattle

文献类型： 外文期刊

作者： Zhou, G. L. 2 ; Zhang, G. L. 1 ; Cao, Y. 1 ; Jin, H. G. 1 ;

作者机构： 1.Jilin Acad Agr Sci, Branch Anim Husb, Gongzhuling 136100, Peoples R China

2.Liaocheng Univ, Coll Life Sci, Liaocheng 252059, Peoples R China

关键词： Malic enzyme gene;Alternative transcripts;Polymorphism;Chinese red cattle;Meat quality and carcass traits

期刊名称：MEAT SCIENCE （ 影响因子：5.209； 五年影响因子：5.305 ）

ISSN： 0309-1740

年卷期： 2011 年 89 卷 1 期

页码：

收录情况： SCI

摘要： The objective of this study was to identify alternative transcripts and single nucleotide polymorphisms (SNPs) in the 3'-untranslated region (3' UTR) of bovine malic enzyme (ME1) gene and to evaluate the extent to which polymorphisms were associated with meat quality and carcass traits in Chinese red cattle. Two transcripts, long transcript and short transcript that differ in the length of the 3' UTR were cloned. A single nucleotide polymorphism was detected in 3' UTR and a restriction site for endonuclease ME1-Dra I was also found. The result revealed that the ME1-Dra I genotypes had a significant effect on cooking loss, pH measured 24 h post-mortem (pH(24h)) and eye muscle area (P<0.05). In conclusion, the SNPs may be used as DNA markers to select for meat quality and carcass traits in Chinese red cattle. (C) 2011 Elsevier Ltd. All rights reserved.

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