文献类型： 外文期刊

作者： Zhu, C. ¹ ; Ye, J. L. ¹ ; Yang, J. ¹ ; Yang, K. M. ² ; Chen, Z. ¹ ; Liang, R. ¹ ; Wu, X. J. ¹ ; Wang, L. ² ; Jiang, Z. Y. ¹ ;

作者机构： 1.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou 510640, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Key Lab Anim Nutr & Feed South China, Inst Anim Sci, Minist Agr PR China, Guangzhou 510640, Guangdong, Peoples R China

3.Guangdong Acad Agr Sci, Key Lab Anim Nutr & Feed South China, Inst Anim Sci, Minist A

关键词： aquaporin;cyclic adenosine monophosphate pathway;diarrhea;enterotoxigenic Escherichia coli K88;ion transporter;piglet

期刊名称：JOURNAL OF ANIMAL SCIENCE （ 影响因子：3.159； 五年影响因子：2.912 ）

ISSN： 0021-8812

年卷期： 2017 年 95 卷 12 期

页码：

收录情况： SCI

摘要： The study was to determine whether the expression of genes involved in intestinal water and ion transport would be affected by enterotoxigenic Escherichia coli (ETEC) K88 both in vitro and in vivo. First, 36 male piglets (4 d old) were randomly allotted to either the control or the ETEC K88 group. Each group had 6 replicates with 3 piglets per replicate. All piglets were fed with the same diets for 17 d. On d 15, piglets in the ETEC K88 group were challenged with ETEC K88 (serotype O149:K91:K88ac) at 1 x 10(8) cfu per pig, whereas those in the control group received the same volume of sterile PBS. After being challenged with ETEC K88 for 72 h (d 18), 1 piglet from each replicate was selected for slaughter to collect samples from the jejunum, ileum, and colon. The mRNA expression and protein abundance of cystic fibrosis transmembrane conductance regulator (CFTR) in the ileum and colon were increased compared with that in the control group (P < 0.05). Furthermore, the mRNA expression of Na-K-Cl cotransporter 1 (NKCC1) in the ileum and colon was increased by ETEC K88 challenge (P < 0.05), whereas in the jejunum, both its mRNA and protein expression were increased by ETEC K88 treatment (P < 0.05). Additionally, an established porcine intestinal epithelial cell line (IPEC-J2) was used to investigate the effect and possible mechanism of ETEC K88 on expression of water channel aquaporins (AQP) and ion transporters. Cells (1.17 x 10(6) per well) were grown in 6-well plates and treated with ETEC K88 at a multiplicity of infection of 50: 1 for 3 h. The mRNA expression of AQP3, AQP11, and Na+/H+ exchanger 3 (NHE3) in IPEC-J2 cells was reduced after ETEC K88 treatment (P < 0.05). Further analyses using western blotting also demonstrated that ETEC K88 decreased the protein expression of AQP3, AQP9, and AQP11 in IPEC-J2 cells (P < 0.05). Moreover, the phosphorylation levels of protein kinase A (PKA) and cyclic adenosine monophosphate (cAMP)-response element binding protein (CREB) were decreased by ETEC K88 challenge (P < 0.05). The results indicate that ETEC K88 challenge induced differential expression of intestinal ion transporters and AQP in young piglets, probably by regulation of the cAMP-PKA signaling pathway. This study might provide new insights about the importance of fluid homeostasis in control of ETEC-induced diarrhea in young piglets.