您好,欢迎访问广东省农业科学院 机构知识库!

De novo Transcriptome Assembly of Chinese Kale and Global Expression Analysis of Genes Involved in Glucosinolate Metabolism in Multiple Tissue

文献类型: 外文期刊

作者: Wu, Shuanghua 1 ; Lei, Jianjun 1 ; Chen, Guoju 1 ; Chen, Hancai 2 ; Cao, Bihao 1 ; Chen, Changming 1 ;

作者机构: 1.South China Agr Univ, Coll Hort, Dept Vegetable Sci, Guangzhou, Guangdong, Peoples R China

2.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangzhou, Guangdong, Peoples R China

关键词: Chinese kale;de novo assembly;RNA-seq;transcriptome;glucosinolate metabolic pathways;gene expression;multiple tissues

期刊名称:FRONTIERS IN PLANT SCIENCE ( 影响因子:5.753; 五年影响因子:6.612 )

ISSN: 1664-462X

年卷期: 2017 年 8 卷

页码:

收录情况: SCI

摘要: Chinese kale, a vegetable of the cruciferous family, is a popular crop in southern China and Southeast Asia due to its high glucosinolate content and nutritional qualities. However, there is little research on the molecular genetics and genes involved in glucosinolate metabolism and its regulation in Chinese kale. In this study, we sequenced and characterized the transcriptomes and expression profiles of genes expressed in 11 tissues of Chinese kale. A total of 216 million 150-bp clean reads were generated using RNA-sequencing technology. From the sequences, 98,180 unigenes were assembled for the whole plant, and 49,582 similar to 98,423 unigenes were assembled for each tissue. Blast analysis indicated that a total of 80,688 (82.18%) unigenes exhibited similarity to known proteins. The functional annotation and classification tools used in this study suggested that genes principally expressed in Chinese kale, were mostly involved in fundamental processes, such as cellular and molecular functions, the signal transduction, and biosynthesis of secondary metabolites. The expression levels of all unigenes were analyzed in various tissues of Chinese kale. A large number of candidate genes involved in glucosinolate metabolism and its regulation were identified, and the expression patterns of these genes were analyzed. We found that most of the genes involved in glucosinolate biosynthesis were highly expressed in the root, petiole, and in senescent leaves. The expression patterns of ten glucosinolate biosynthetic genes from RNA-seq were validated by quantitative RT-PCR in different tissues. These results provided an initial and global overview of Chinese kale gene functions and expression activities in different tissues.

  • 相关文献

[1]Comparative Transcriptome Analysis Reveals Differential Transcription in Heat-susceptible and Heat-tolerant Pepper (Capsicum annum L.) Cultivars under Heat Stress. Li, Tao,Xu, Xiaowan,Li, Ying,Wang, Hengming,Li, Zhiliang,Li, Zhenxing,Li, Tao,Xu, Xiaowan.

[2]De novo assembly, gene annotation, and marker development of mulberry (Morus atropurpurea) transcriptome. Dai, Fanwei,Tang, Cuiming,Wang, Zhenjiang,Luo, Guoqing,He, Li,Yao, Liuhui. 2015

[3]Transcriptome comparison in the pituitary-adrenal axis between Beagle and Chinese Field dogs after chronic stress exposure. Luo, Wei,Xu, Haiping,Nie, Qinghua,Luo, Wei,Xu, Haiping,Nie, Qinghua,Luo, Wei,Xu, Haiping,Nie, Qinghua,Fang, Meixia,Xing, Huijie.

[4]Molecular Characterization of MYB28 Involved in Aliphatic Glucosinolate Biosynthesis in Chinese Kale (Brassica oleracea var. alboglabra Bailey). Yin, Ling,Cao, Bihao,Lei, Jianjun,Chen, Guoju,Chen, Hancai. 2017

[5]基于RNA-seq筛选影响猪肉品质的关键候选基因. 肖明飞,申露露,何逸懿,易宏波,唐青松,李莉,洪兴,李大江,徐娥. 2022

[6]辣椒杂交种与亲本苗期叶片基因差异表达分析. 徐小万,李颖,王恒明,李涛,何水林,官德义. 2016

[7]玫烟色棒束孢诱导的小菜蛾免疫响应表达谱分析. 雷妍圆,何余容,谢梅琼,吕利华,顾家睿. 2016

[8]RNA-Seq Analyses for Two Silkworm Strains Reveals Insight into Their Susceptibility and Resistance to Beauveria bassiana Infection. Xing, Dongxu,Jiang, Liang,Xia, Qingyou,Xing, Dongxu,Yang, Qiong,Li, Qingrong,Xiao, Yang,Ye, Mingqiang. 2017

[9]Transcriptome profiling of resistant and susceptible Cavendish banana roots following inoculation with Fusarium oxysporum f. sp cubense tropical race 4. Li, Chun-yu,Jin, Yan,Kuang, Rui-bin,Yang, Qiao-song,Sheng, Ou,Wei, Yue-rong,Hu, Chun-hua,Dong, Tao,Yi, Gan-jun,Li, Chun-yu,Jin, Yan,Kuang, Rui-bin,Yang, Qiao-song,Sheng, Ou,Wei, Yue-rong,Hu, Chun-hua,Dong, Tao,Yi, Gan-jun,Deng, Gui-ming,Yang, Jing,Zuo, Cun-wu,Lv, Zhi-cheng,Viljoen, Altus. 2012

[10]GADD45 beta, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens. Zhang, Xinheng,Yan, Zhuanqiang,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Zhang, Xinheng,Yan, Zhuanqiang,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Zhang, Xinheng,Yan, Zhuanqiang,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Zhang, Xinheng,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Lin, Wencheng,Chen, Weiguo,Chen, Feng,Xie, Qingmei,Zhang, Huanmin. 2016

[11]Comparative transcriptome analysis of sweet corn seedlings under low-temperature stress. Mao, Jihua,Yu, Yongtao,Yang, Jing,Li, Gaoke,Li, Chunyan,Qi, Xitao,Wen, Tianxiang,Hu, Jianguang. 2017

[12]Transcriptome sequencing reveals genetic mechanisms underlying the transition between the laying and brooding phases and gene expression changes associated with divergent reproductive phenotypes in chickens. Shen, Xu,Shen, Xu,Shen, Xu,Xu, Haipin,Nie, Qinghua,Zhang, Xiquan,Shen, Xu,Xu, Haipin,Nie, Qinghua,Zhang, Xiquan,Shen, Xu,Bai, Xue,Xu, Jin,Lu, Xuemei,Zhou, Min.

[13]Transcriptional profiling of the responses to infection by the false smut fungus Ustilaginoidea virens in resistant and susceptible rice varieties. Yang, Chao,Li, Luoye,Li, Jianxiong,Feng, Aiqing,Zhu, Xiaoyuan.

[14]Transcriptome-wide sequencing provides insights into geocarpy in peanut (Arachis hypogaea L.). Li, Haifen,Hong, Yanbin,Zhu, Fanghe,Zhu, Wei,Liu, Haiyan,Zhang, Erhua,Zhong, Ni,Wen, Shijie,Li, Xingyu,Zhou, Guiyuan,Li, Shaoxiong,Varshney, Rajeev,Liang, Xuanqiang,Yang, Qingli,Pan, Lijuan,Chen, Na,Chi, Xiaoyuan,Chen, Mingna,Yang, Zhen,Wang, Tong,Wang, Mian,Yu, Shanlin,Yang, Qingli,Li, Heying,Liu, Hong,Wu, Hong,Varshney, Rajeev.

[15]Achievements and prospects of genomics-assisted breeding in three legume crops of the semi-arid tropics. Varshney, Rajeev K.,Mohan, S. Murali,Gaur, Pooran M.,Pandey, Manish K.,Sawargaonkar, Shrikant L.,Chitikineni, Annapurna,Janila, Pasupuleti,Saxena, K. B.,Sharma, Mamta,Rathore, Abhishek,Mallikarjuna, Nalini,Gowda, C. L. L.,Varshney, Rajeev K.,Varshney, Rajeev K.,Varshney, Rajeev K.,Liang, Xuanqiang,Gangarao, N. V. P. R.,Pandey, Manish K.,Bohra, Abhishek,Pratap, Aditya,Datta, Subhojit,Chaturvedi, S. K.,Nadarajan, N.,Kimurto, Paul K.,Fikre, Asnake,Tripathi, Shailesh,Bharadwaj, Ch.,Anuradha, G.,Babbar, Anita,Choudhary, Arbind K.,Mhase, M. B.,Mannur, D. M.. 2013

[16]De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction. Liu, Chuan-He,Fan, Chao,Liu, Chuan-He,Fan, Chao. 2016

[17]Transcriptomic Analysis of Seed Coats in Yellow-Seeded Brassica napus Reveals Novel Genes That Influence Proanthocyanidin Biosynthesis. Hong, Meiyan,Hu, Kaining,Tian, Tiantian,Li, Xia,Chen, Li,Zhang, Yan,Yi, Bin,Wen, Jing,Ma, Chaozhi,Shen, Jinxiong,Fu, Tingdong,Tu, Jinxing,Li, Xia,Chen, Li,Zhang, Yan. 2017

[18]Temporal patterns of gene expression associated with tuberous root formation and development in sweetpotato (Ipomoea batatas). Wang, Zhangying,Fang, Boping,Chen, Xinliang,Liao, Minghuan,Chen, Jingyi,Zhang, Xiongjian,Huang, Lifei,Luo, Zhongxia,Yao, Zhufang,Li, Yujun. 2015

[19]Analysis of Muscle and Ovary Transcriptome of Sus scrofa: Assembly, Annotation and Marker Discovery. Nie, Qinghua,Jia, Xinzheng,Zhang, Wei,Zhou, Xiaoning,He, Xiaomei,Zhang, Xiquan,Nie, Qinghua,Jia, Xinzheng,Zhang, Wei,Zhou, Xiaoning,He, Xiaomei,Zhang, Xiquan,Fang, Meixia. 2011

[20]Phenotypic and Transcriptomic Analyses of Autotetraploid and Diploid Mulberry (Morus alba L.). Dai, Fanwei,Wang, Zhenjiang,Luo, Guoqing,Tang, Cuiming. 2015

作者其他论文 更多>>