您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effects of Incubation Conditions on Cr(VI) Reduction by c-type Cytochromes in Intact Shewanella oneidensis MR-1 Cells

文献类型： 外文期刊

作者： Han, Rui 1 ; Li, Fangbai 2 ; Liu, Tongxu 2 ; Li, Xiaomin 2 ; Wu, Yundang 2 ; Wang, Ying 2 ; Chen, Dandan 2 ;

作者机构： 1.South China Univ Technol, Sch Environm & Energy, Guangzhou, Guangdong, Peoples R China

2.Guangdong Inst Ecoenvironm & Soil Sci, Guangdong Key Lab Agr Environm Pollut Integrated, Guangzhou, Guangdong, Peoples R China

关键词： c-type cytochromes;Shewanella oneidensis MR-1;Cr(VI) reduction;intact cells;incubation conditions

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： It is widely recognized that the outer membrane c-type cytochromes (OM c-Cyts) of metal-reducing bacteria play a key role in microbial metal reduction processes. However, the in situ redox status of OM c-Cyts during microbial metal reduction processes remain poorly understood. In this study, diffuse-transmission UV/Vis spectroscopy is used to investigate the in situ spectral reaction of Cr(VI) reduction by c-Cyts in intact Shewanella oneidensis MR-1 cells under different incubation conditions. The reduced c-Cyts decreased transiently at the beginning and then recovered gradually over time. The Cr(VI) reduction rates decreased with increasing initial Cr(VI) concentrations, and Cr(III) was identified as a reduced product. The presence of Cr(III) substantially inhibited Cr(VI) reduction and the recovery of reduced c-Cyts, indicating that Cr(III) might inhibit cell growth. Cr(VI) reduction rates increased with increasing cell density. The highest Cr(VI) reduction rate and fastest recovery of c-Cyts were obtained at pH 7.0 and 30 degrees C, with sodium lactate serving as an electron donor. The presence of 02 strongly inhibited Cr(VI) reduction, suggesting that 02 might compete with Cr(VI) as an electron acceptor in cells. This study provides a case of directly examining in vivo reaction properties of an outer-membrane enzyme during microbial metal reduction processes under non-invasive physiological conditions.

  • 相关文献

[1]In-situ Cr(VI) reduction with electrogenerated hydrogen peroxide driven by iron-reducing bacteria. Liu, Liang,Yuan, Yong,Li, Fang-bai,Liu, Liang,Feng, Chun-hua,Liu, Liang.

[2]In situ spectral kinetics of quinone reduction by c-type cytochromes in intact Shewanella oneidensis MR-1 cells. Han, Rui,Li, Xiaomin,Wu, Yundang,Li, Fangbai,Liu, Tongxu,Han, Rui. 2017

[3]Axial Ligation of Heme in c-Type Cytochromes of Living Shewanella oneidensis: A New Insight into Enhanced Extracellular Electron Transfer. Yuan, Yong,Zhou, Shungui,Li, Laicai. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台