Identification of a New Sprouty Protein Responsible for the Inhibition of the Bombyx mori Nucleopolyhedrovirus Reproduction
文献类型： 外文期刊
作者： Jin, Shengkai 1 ; Cheng, Tingcai 1 ; Jiang, Liang 1 ; Lin, Ping 1 ; Yang, Qiong 2 ; Xiao, Yang 2 ; Kusakabe, Takahiro; 1 ;
作者机构： 1.Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing, Peoples R China
2.Guangdong Acad Agr Sci, Sericulture & Farm Prod Proc Res Inst, Guangzhou, Guangdong, Peoples R China
3.Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Lab Silkworm Sci, Fukuoka 812, Japan
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2014 年 9 卷 6 期
页码：
收录情况： SCI
摘要： The rat sarcoma-extracellular signal regulated kinase mitogen-activated protein kinases pathway, one of the most ancient signaling pathways, is crucial for the defense against Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Sprouty (Spry) proteins can inhibit the activity of this pathway by receptor tyrosine kinases. We cloned and identified a new B. mori gene with a Spry domain similar to the Spry proteins of other organisms, such as fruitfly, mouse, human, chicken, Xenopus and zebrafish, and named it BmSpry. The gene expression analysis showed that BmSpry was transcribed in all of the examined tissues and in all developmental stages from embryo to adult. BmSpry also induced expression of BmNPV in the cells. Our results indicated: (1) the knock-down of BmSpry led to increased BmNPV replication and silkworm larvae mortality; (2) overexpression of BmSpry led to reduced BmNPV replication; and (3) BmSpry regulated the activation of ERK and inhibited BmNPV replication. These results showed that BmSpry plays a crucial role in the antiviral defense of the silkworm both in vitro and in vivo.
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