您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Metabolic engineering of tomato for high-yield production of astaxanthin

文献类型： 外文期刊

作者： Huang, Jun-Chao 1 ; Zhong, Yu-Juan 2 ; Liu, Jin 2 ; Sandmann, Gerhard 4 ; Chen, Feng 5 ;

作者机构： 1.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China

2.Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China

3.Guangdong Acad Agr Sci, Inst Vegetable Res, Guangzhou, Guangdong, Peoples R China

4.Goethe Univ Frankfurt, Biosynth Grp, D-60054 Frankfurt, Germany

5.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China

关键词： Astaxanthin;beta-carotene ketolase;beta-carotene hydroxylase;Tomato

期刊名称：METABOLIC ENGINEERING （ 影响因子：9.783； 五年影响因子：9.566 ）

ISSN： 1096-7176

年卷期： 2013 年 17 卷

页码：

收录情况： SCI

摘要： Dietary carotenoids have been shown to be beneficial to health by decreasing the risk of many diseases. Attempts to enhance carotenoids in food crops have been successful although higher plants appear to resist big changes of carotenoid biosynthesis by metabolic engineering. Here we report the generation of a more nutritious tomato by modifying the intrinsic carotenes to astaxanthin, a high-value ketocarotenoid rarely found in plants. This was achieved by co-expression of the algal beta-carotene ketolase from Chlamydomonas reinhardtii and beta-carotene hydroxylase from Haematococcus pluvialis, a unique pair of enzymes identified to co-operate perfectly in converting p-carotene to astaxanthin by functional complementation in Escherichia coli. Expression of the two enzymes in tomato up-regulated most intrinsic carotenogenic genes, and efficiently directed carbon flux into carotenoids, leading to massive accumulations of mostly free astaxanthin in leaves (3.12 mg/g) but esterified astaxanthin in fruits (16.1 mg/g) and a 16-fold increase of total carotenoid capacity therein without affecting the plant normal growth and development. This study opened up the possibility of employing crop plants as green factories for economical production of astaxanthin. (C) 2013 Elsevier Inc. All rights reserved.

  • 相关文献

[1]Clothianidin dissipation in tomato and soil, and distribution in tomato peel and flesh. Li, Li,Jiang, Gaiqing,Liang, Hongwu,Sun, Dali,Li, Wei,Liu, Congyun,Liang, Hongwu. 2012

[2]Seasonal variation in the biocontrol efficiency of bacterial wilt is driven by temperature-mediated changes in bacterial competitive interactions. Huang, Jianfeng,Yang, Tianjie,Jousset, Alexandre,Xu, Yangchun,Shen, Qirong,Huang, Jianfeng,Yang, Tianjie,Jousset, Alexandre,Friman, Ville-Petri.

[3]Pyrimethanil residue and dissipation in tomatoes and soil under field conditions. Liu, Congyun,Lu, Dahai,Wan, Kai,Huang, Jianxiang,Wang, Fuhua,Liu, Congyun,Lu, Dahai,Wan, Kai,Huang, Jianxiang,Wang, Fuhua,Wang, Youcheng.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台