您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Analysis of long noncoding RNAs and messenger RNAs expression profiles in the hearts of mice with acute viral myocarditis

文献类型： 外文期刊

作者： Xue, Yimin 1 ; Ke, Jun 2 ; Zhang, Jiuyun 2 ; Chen, Mingguang 1 ; Zeng, Lijuan 1 ; Fan, Qiaolian 1 ; Zheng, Chunfu 4 ; Chen, Feng 2 ;

作者机构： 1.Fujian Med Univ, Fujian Prov Hosp, Dept Crit Care Med 4, Shengli Clin Med Coll, Fuzhou, Fujian, Peoples R China

2.Fujian Med Univ, Fujian Prov Hosp, Dept Emergency, Shengli Clin Med Coll, Fuzhou, Fujian, Peoples R China

3.Fujian Prov Hosp, Fujian Prov Key Lab Emergency Med, Fuzhou, Fujian, Peoples R China

4.Guangdong Acad Agr Sci, Key Lab Livestock Dis Prevent Guangdong Prov, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Minist Agr & Rural Affairs,Inst Anim Hlth,Guangdon, Guangzhou, Guangdong, Peoples R China

5.Univ Calgary, Dept Microbiol Immunol & Infect Dis, Calgary, AB, Canada

6.Fujian Med Univ, Fujian Prov Hosp, Dept Emergency, Shengli Clin Med Coll, 134 East St, Fuzhou 350001, Fujian, Peoples R China

关键词： acute viral myocarditis; coxsackievirus B3; long noncoding RNAs; messenger RNAs; RNA sequencing

期刊名称：JOURNAL OF MEDICAL VIROLOGY （ 影响因子：12.7； 五年影响因子：8.5 ）

ISSN： 0146-6615

年卷期： 2023 年 95 卷 2 期

页码：

收录情况： SCI

摘要： Acute viral myocarditis (AVMC) is a common acute myocardial inflammation caused by viral infections, which can lead to severe cardiac dysfunction. Several long noncoding RNAs (lncRNAs) with aberrant expression have been identified in the pathogenesis of AVMC. However, the expression profiles and functions of lncRNAs in AVMC have not been fully elucidated. In the present study, we constructed AVMC mouse models by intraperitoneal injection of coxsackievirus B3 (CVB3) and performed RNA sequencing (RNA-seq) on heart tissues to investigate the differences in lncRNAs and messenger RNAs (mRNAs) expression profiles. Based on the cutoff criteria of adjusted p-values (padj) 1, a total of 1122 differentially expressed lncRNAs (DElncRNAs) and 3186 differentially expressed mRNAs (DEmRNAs) were screened, including 734 upregulated and 388 downregulated lncRNAs, 1821 upregulated and 1365 downregulated mRNAs. RT-qPCR analysis validated that the expression patterns of 12 randomly selected genes (6 DElncRNAs and 6 DEmRNAs) were highly consistent with those in RNA-seq, proving the reliability of the RNA-seq data. Then, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that differentially expressed genes were mainly involved in metabolic and immune-related processes. Furthermore, co-expression networks between DElncRNAs and DEmRNAs in cytokine-cytokine receptor interaction, MAPK signaling pathway, and PI3K-Akt signaling pathway were constructed to study the molecular interactions of these molecules. Our study, for the first time, reveals the expression profiles of lncRNAs and mRNAs associated with AVMC, which may shed light on the roles of lncRNAs in disease pathogenesis and aid in discovering new therapeutic targets.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台