文献类型： 外文期刊

作者： Yu, Changqing ¹ ; Bai, Yuanzhe ² ; Tan, Wenbo ³ ; Bai, Yu ⁴ ; Li, Xuemei ¹ ; Zhou, Yulong ⁵ ; Zhai, Jingbo ⁶ ; Xue, Mengzhou ⁷ ; Tang, Yan-Dong ² ; Zheng, Chunfu ⁸ ; Liu, Qiang ¹⁰ ;

作者机构： 1.Yibin Vocat & Tech Coll, Engn Ctr Agr Biosafety Assessment & Biotechnol, Sch Adv Agr Sci, Yibin, Peoples R China

2.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Harbin, Peoples R China

3.Heilongjiang Univ, Coll Adv Agr & Ecol Environm, Harbin, Peoples R China

4.Wenzhou Vocat Coll Sci & Technol, Coll Anim Sci, Wenzhou, Peoples R China

5.Heilongjiang Bayi Agr Univ, Coll Anim Sci & Technol, Daqing, Peoples R China

6.Univ Inner Mongolia Autonomous Reg, Inner Mongolia Minzu Univ, Med Coll, Key Lab Zoonose Prevent & Control, Tongliao, Peoples R China

7.Zhengzhou Univ, Affiliated Hosp 2, Dept Cerebrovascular Dis, Zhengzhou, Peoples R China

8.Guangdong Acad Agr Sci, Key Lab Livestock Dis Prevent Guangdong Prov, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Minist Agr & Rural Affairs,Inst Anim Hlth, Guangzhou, Peoples R China

9.Univ Calgary, Dept Microbiol Immunol & Infect Dis, Calgary, AB, Canada

10.Nanchong Vocat & Tech Coll, Nanchong Key Lab Dis Prevent Control & Detect Live, Nanchong, Peoples R China

11.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Haping Rd 678, Harbin 150069, Peoples R China

12.Univ Calgary, Dept Microbiol Immunol & Infect Dis, Calgary, AB T2N 4N1, Canada

13.Nanchong Vocat & Tech Coll, 100 Xiaoxi Rd, Nanchong 637131, Sichuan, Peoples R China

关键词： cleavage; Ebola virus; envelope glycoprotein; furin; MARCH

期刊名称：JOURNAL OF MEDICAL VIROLOGY （ 影响因子：12.7； 五年影响因子：8.5 ）

ISSN： 0146-6615

年卷期： 2024 年 96 卷 2 期

页码：

收录情况： SCI

摘要： Membrane-associated RING-CH (MARCH) family proteins were recently reported to inhibit viral replication through multiple modes. Previous work showed that human MARCH8 blocked Ebola virus (EBOV) glycoprotein (GP) maturation. Our study here demonstrates that human MARCH1 and MARCH2 share a similar pattern to MARCH8 in restricting EBOV GP-pseudotyped viral infection. Human MARCH1 and MARCH2 retain EBOV GP at the trans-Golgi network, reduce its cell surface display, and impair EBOV GP-pseudotyped virions infectivity. Furthermore, we uncover that the host proprotein convertase furin could interact with human MARCH1/2 and EBOV GP intracellularly. Importantly, the furin P domain is verified to be recognized by MARCH1/2/8, which is critical for their blocking activities. Besides, bovine MARCH2 and murine MARCH1 also impair EBOV GP proteolytic processing. Altogether, our findings confirm that MARCH1/2 proteins of different mammalian origins showed a relatively conserved feature in blocking EBOV GP cleavage, which could provide clues for subsequent MARCHs antiviral studies and may facilitate the development of novel strategies to antagonize enveloped virus infection.