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Intraspecific polymorphism of rDNA among five Nosema bombycis isolates from different geographic regions in China

文献类型： 外文期刊

作者： Liu, Handeng 1 ; Pan, Guoqing 1 ; Luo, Bo 1 ; Li, Tian 1 ; Yang, Qiong 4 ; Vossbrinck, Charles R. 5 ; Debrunner-Vossbr 1 ;

作者机构： 1.Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing 400716, Peoples R China

2.Chongqing Med Univ, Expt Teaching Ctr, Chongqing 400016, Peoples R China

3.Chongqing Normal Univ, Lab Anim Biol, Chongqing 400047, Peoples R China

4.Guangdong Acad Agr Sci, Sericulture & Farm Produce Proc Res Inst, Guangzhou 510610, Guangdong, Peoples R China

5.Connecticut Agr Expt Stn, New Haven, CT 06504 USA

6.Gateway Community Coll, Dept Biol, New Haven, CT 06504 USA

关键词： Nosema bombycis;Genetic polymorphism;Geographic differentiation;rDNA;Intergenic region

期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.841； 五年影响因子：3.368 ）

ISSN： 0022-2011

年卷期： 2013 年 113 卷 1 期

页码：

收录情况： SCI

摘要： The microsporidian Nosema bombycis is the causative agent of pebrine, a highly infectious disease of the silkworm Bombyx mori. Three regions of the multicopy rDNA gene were examined in order to investigate the relationships among five Nosema isolates from various regions of China. Ribosomal DNA alleles are present on each of the 18 chromosomes of N. bombycis and show a high degree of variation. In this study the small subunit (SSU) rDNA, internal transcribed spacer (ITS) and intergenic spacer (IGS) regions for up to 10 different rDNA copies from each N. bombycis isolate were cloned and sequenced. As expected we see greater polymorphism in the ITS region (88 variable sites in 179 nucleotides) and IGS (200 variable sites in 279 nucleotides) than in the SSU rDNA (24 variable sites in 1232 nucleotides). Phylogenetic analysis shows greater differences between alleles within an isolate than between the same alleles from different isolates. The data reveal two very different groups, one from the Sichuan province and the other with a broad distribution including four provinces in southeast China and Japan. The Sichuan isolate does not have any rDNA alleles with sequences identical to those in the other isolates, implying that it is a separate, non-intermixing, population or perhaps a separate species from the other isolates. In light of the polymorphic nature of the rDNA alleles in N. bombycis and their presence on every chromosome, the rDNA gene may be useful for understanding the movement and ultimately the source of pebrine infections. (c) 2013 Elsevier Inc. All rights reserved.

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