Vaccination with porcine reproductive and respiratory syndrome killed virus vaccine and immunostimulatory oligodeoxynucleotides induces specitic immunity in piglets
文献类型: 外文期刊
作者: Zhang Linghua 1 ; Tian Xingshan 2 ; Zhou Fengzhen 2 ;
作者机构: 1.S China Agr Univ, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China
2.S China Agr Univ, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China; Guangdong Acad Agr Sci, Guangzhou 510642, Guangdong, Peoples R China
关键词: the piglets;porcine reproductive and respiratory syndrome killed virus vaccine;CpG ODN;immune response
期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )
ISSN: 0264-410X
年卷期: 2007 年 25 卷 10 期
页码:
收录情况: SCI
摘要: A large number of studies demonstrated the immunostimulatory effects of CpG oligonucleotides (ODN), particularly in mice. In present study, the objective was to investigate the immunoadjuvant effects of CpG ODN to porcine reproductive and respiratory syndrome (PRRS) killed virus vaccine (PRRSV) and its protective effects against PRRS virus in piglets. The PRRSV-specific antibodies titres and serum IgG1/IgG2 titres, the proliferation of lymphocytes, PRRSV-specific IFN-gamma, the expression of major histocompatibility complex class II (MHCII) of peripheral blood mononuclear cells (PBMCs) and post-challenge clinical protection were examined to identify the immune responses of the piglets. The results were found that the above-mentioned immune responses of the piglets inoculated with CpG ODN plus PRRSV were significantly stronger than those indued by PRRSV or PBS control groups. All these data suggested that CpG ODN could be employed as effective immunoadjuvant to raise the Immoral and cellular responses of the piglets to PRRSV. (c) 2006 Elsevier Ltd. All rights reserved.
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