In vivo effects of oligodeoxynucleotides containing synthetic immunostimulatory motifs in the immune response to swine streptococcic septicemia vaccine in weaned piglets
文献类型: 外文期刊
作者: Zhang Linghua 1 ; Tian Xingshan 2 ; Zhou Fengzhen 2 ;
作者机构: 1.S China Agr Univ, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China
2.S China Agr Univ, Coll Life Sci, Guangzhou 510642, Guangdong, Peoples R China; Guangdong Acad Agr Sci, Biotech Res Inst, Guangzhou 510640, Peoples R China
关键词: CpG ODN;immune response;the weaned piglets;swine streptococcic septicemia killed vaccine
期刊名称:MOLECULAR IMMUNOLOGY ( 影响因子:4.407; 五年影响因子:4.227 )
ISSN: 0161-5890
年卷期: 2007 年 44 卷 6 期
页码:
收录情况: SCI
摘要: The diverse immunostimulatory effects of CpG oligodeoxynucleotides (CpG ODN) have been demonstrated extensively in mice and human. Although the immunoadjuvant effects of CpG ODN in pigs were also studied in several reports, until now, little work has been carried out with regard to their effects on the adaptive immune system of newly weaned piglets. In this study, swine streptococcic septicemia killed vaccine (SSSK vaccine) was used as antigen, we assessed the in vivo immunostimulatory effects of different CpG motifs in newly weaned piglets. The proportion of CD4(+), CD8(+) T lymphocytes subpopulations and proliferation of peripheral blood mononuclear cells (PBMCs), IFN-gamma and IL-6 in serum, and the titre of IgG and IgG2/IgG1 isotype to SSSK vaccine in serum were tested at different time-points. The results suggested that, the CD4(+)/CD8(+) ratio decreased significantly in weaned piglets inoculated with phosphate buffer saline (PBS) alone, however, it was stable in CpG ODN-coinoculated newly weaned piglets. IFN-gamma and IL-6 levels, the titres of specific antibodies IgG, IgG2 and proliferative responses of CpG ODN-coinjected piglets were all significantly higher than those of SSSK vaccine alone or PBS or GpC ODN-coinjected piglets. The porcine-specific ODN-induced responses were stronger in animals injected with human-specific or mouse-specific CpG ODN. These in vivo data demonstrate for the first time that CpG ODN can stimulate adaptive immune system in weaned piglets. (c) 2006 Elsevier Ltd. All rights reserved.
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