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RNA interference of chitin synthase genes inhibits chitin biosynthesis and affects larval performance in Leptinotarsa decemlineata (Say)

文献类型： 外文期刊

作者： Shi, Ji-Feng 1 ; Mu, Li-Li 1 ; Chen, Xu 1 ; Guo, Wen-Chao 2 ; Li, Guo-Qing 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Educ Minist, Nanjing 210095, Jiangsu, Peoples R China

2.Xinjiang Acad Agr Sci, Dept Plant Protect, Urumqi 830091, Peoples R China

关键词： Leptinotarsa decemlineata;chitin synthase;RNA interference;control

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES （ 影响因子：6.58； 五年影响因子：6.478 ）

ISSN： 1449-2288

年卷期： 2016 年 12 卷 11 期

页码：

收录情况： SCI

摘要： Dietary introduction of bacterially expressed double-stranded RNA (dsRNA) has great potential for management of Leptinotarsa decemlineata. Identification of the most attractive candidate genes for RNA interference (RNAi) is the first step. In the present paper, three complete chitin synthase cDNA sequences (LdChSAa, LdChSAb and LdChSB) were cloned. LdChSAa and LdChSAb, two splicing variants of LdChSA gene, were highly expressed in ectodermally-derived epidermal cells forming epidermis, trachea, foregut and hindgut, whereas LdChSB was mainly transcribed in midgut cells. Feeding bacterially expressed dsChSA (derived from a common fragment of LdChSAa and LdChSAb), dsChSAa, dsChSAb and dsChSB in the second-and fourth-instar larvae specifically knocked down their target mRNAs. RNAi of LdChSAa+LdChSAb and LdChSAa lowered chitin contents in whole body and integument samples, and thinned tracheal taenidia. The resulting larvae failed to ecdyse, pupate, or emerge as adults. Comparably, knockdown of LdChSAb mainly affected pupal-adult molting. The LdChSAb RNAi pupae did not completely shed the old larval exuviae, which caused failure of adult emergence. In contrast, silencing of LdChSB significantly reduced foliage consumption, decreased chitin content in midgut sample, damaged midgut peritrophic matrix, and retarded larval growth. As a result, the development of the LdChSB RNAi hypomorphs was arrested. Our data reveal that these LdChSs are among the effective candidate genes for an RNAi-based control strategy against L. decemlineata.

  • 相关文献

[1]IDENTIFICATION AND HORMONE INDUCTION OF PUTATIVE CHITIN SYNTHASE GENES AND SPLICE VARIANTS IN Leptinotarsa decemlineata (SAY). Shi, Ji-Feng,Mu, Li-Li,Li, Guo-Qing,Guo, Wen-Chao.

[2]Knockdown of juvenile hormone acid methyl transferase severely affects the performance of Leptinotarsa decemlineata (Say) larvae and adults. Fu, Kai-Yun,Li, Qian,Zhou, Li-Tao,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[3]Physiological roles of trehalose in Leptinotarsa larvae revealed by RNA interference of trehalose-6-phosphate synthase and trehalase genes. Shi, Ji-Feng,Xu, Qing-Yu,Sun, Qiang-Kun,Meng, Qing-Wei,Mu, Li-Li,Li, Guo-Qing,Guo, Wen-Chao.

[4]Identification of ten mevalonate enzyme-encoding genes and their expression in response to juvenile hormone levels in Leptinotarsa decemlineata (Say). Li, Qian,Meng, Qing-Wei,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[5]CHARACTERIZATION AND FUNCTIONAL STUDY OF A PUTATIVE JUVENILE HORMONE DIOL KINASE IN THE COLORADO POTATO BEETLE Leptinotarsa decemlineata (Say). Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[6]Characterization of two juvenile hormone epoxide hydrolases by RNA interference in the Colorado potato beetle. Lu, Feng-Gong,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao.

[7]The subunit gene Ld alpha 1 of nicotinic acetylcholine receptors plays important roles in the toxicity of imidacloprid and thiamethoxam against Leptinotarsa decemlineata. Qu, Yang,Chen, Jinhua,Li, Chenge,Wang, Qiang,Han, Zhaojun,Jiang, Weihua,Guo, Wenchao.

[8]Involvement of a putative allatostatin in regulation of juvenile hormone titer and the larval development in Leptinotarsa decemlineata (Say). Meng, Qing-Wei,Liu, Xin-Ping,Lu, Feng-Gong,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao. 2015

[9]Knocking down a putative Delta(1)-pyrroline-5-carboxylate dehydrogenase gene by RNA interference inhibits flight and causes adult lethality in the Colorado potato beetle Leptinotarsa decemlineata (Say). Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Wang, Xin-Xin,Li, Guo-Qing,Wan, Pin-Jun,Guo, Wen-Chao.

[10]Knockdown of a putative alanine aminotransferase gene affects amino acid content and flight capacity in the Colorado potato beetle Leptinotarsa decemlineata. Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao,Wan, Pin-Jun.

[11]A putative Delta(1)-pyrroline-5-carboxylate synthetase involved in the biosynthesis of proline and arginine in Leptinotarsa decemlineata. Wan, Pin-Jun,Fu, Kai-Yun,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[12]Instar-dependent systemic RNA interference response in Leptinotarsa decemlineata larvae. Guo, Wen-Chao,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao,Yang, Shuai,Li, Xiao-Xu.

[13]RNA interference suppression of the receptor tyrosine kinase Torso gene impaired pupation and adult emergence in Leptinotarsa decemlineata. Zhu, Tao-Tao,Meng, Qing-Wei,Li, Guo-Qing,Guo, Wen-Chao.

[14]Transcription response of three putative trehalase genes to hormonal stimulation in the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Shi, Ji-Feng,Sun, Qiang-Kun,Mu, Li-Li,Li, Guo-Qing,Guo, Wen-Chao.

[15]Chlorantraniliprole susceptibility in leptinotarsa decemlineata in the north Xinjiang Uygur autonomous region in China. Jiang, Wei-Hua,Lu, Wei-Ping,Li, Guo-Qing,Guo, Wen-Chao,Xia, Zhen-Han,Fu, Wen-Jun.

[16]Leptinotarsa cap 'n' collar isoform C/Kelch-like ECH associated protein 1 signaling is critical for the regulation of ecdysteroidogenesis in the larvae. Sun, Qiang-Kun,Meng, Qing-Wei,Xu, Qing-Yu,Deng, Pan,Li, Guo-Qing,Guo, Wen-Chao.

[17]Functions of nuclear receptor HR3 during larval-pupal molting in Leptinotarsa decemlineata (Say) revealed by in vivo RNA interference. Guo, Wen-Chao,Liu, Xin-Ping,Fu, Kai-Yun,Shi, Ji-Feng,Lu, Feng-Gong,Li, Guo-Qing,Guo, Wen-Chao.

[18]Response of the vacuolar ATPase subunit E to RNA interference and four chemical pesticides in Leptinotarsa decemlineata (Say). Fu, Kai-Yun,Lu, Feng-gong,Liu, Xin-ping,Li, Guo-Qing,Guo, Wen-Chao.

[19]Involvement of FTZ-F1 in the regulation of pupation in Leptinotarsa decemlineata (Say). Liu, Xin-Ping,Fu, Kai-Yun,Lu, Feng-Gong,Meng, Qing-Wei,Li, Guo-Qing,Guo, Wen-Chao.

[20]Insecticide resistance status of Colorado potato beetle (Coleoptera: Chrysomelidae) adults in Northern Xinjiang Uygur autonomous region. Jiang, Wei-Hua,Wang, Zhi-Tian,Xiong, Man-Hui,Lu, Wei-Ping,Liu, Ping,Li, Guo-Qing,Jiang, Wei-Hua,Wang, Zhi-Tian,Xiong, Man-Hui,Lu, Wei-Ping,Liu, Ping,Li, Guo-Qing,Guo, Wen-Chao.

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