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Identification of cytochrome P450 monooxygenase genes and their expression profiles in cyhalothrin-treated Colorado potato beetle, Leptinotarsa decemlineata

文献类型： 外文期刊

作者： Wan, Pin-Jun 1 ; Shi, Xiao-Qin 1 ; Kong, Ye 1 ; Zhou, Li-Tao 1 ; Guo, Wen-Chao 2 ; Ahmat, Tursun 2 ; Li, Guo-Qing 1 ;

作者机构： 1.Nanjing Agr Univ, Key Lab Integrated Management Crop Dis & Pests, Educ Minist, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

2.Xinjiang Acad Agr Sci, Dept Plant Protect, Urumqi 830091, Peoples R China

关键词： Cytochrome P450 monooxygenase;Detoxification;Insecticide resistance;Enzyme inhibitor

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：3.963； 五年影响因子：4.454 ）

ISSN： 0048-3575

年卷期： 2013 年 107 卷 3 期

页码：

收录情况： SCI

摘要： Based on a Leptinotarsa decemlineata transcriptome dataset and the GenBank sequences, a total of 74 cytochrome P450 monooxygenase genes (Cyps) were identified. These genes fell into CYP2 clan, mitochondrial clan, CYP3 clan and CYP4 clan, and were classified into 19 families and 35 subfamilies according to standard nomenclature. Two new families were discovered in CYP4 clan, and were named CYP412 and CYP413 respectively. Four new families that were recently discovered in Tribolium castaneum, including mitochondrial family CYP353, CYP3 clan families CYP345 and CYP347, and CYP4 clan family CYP350, were also found in L decemlineata. The phylogenetic trees of CYPs from L decemlineata and other representative insect species were constructed, and these trees provided evolutionary insight for the genetic distance. Our results facilitate further researches to understand the functions and evolution of L decemlineata Cyp genes. In order to find cyhalothrin-inducible Cyp genes, the expression levels of Cyps belonging to CYP12, CYP6, CYP9 and CYP4 families were determined by quantitative reverse transcriptase-PCR in cyhalothrin-treated and control fourth-instar larvae. Nine Cyp genes, i.e., Cyp12H2, Cyp6BH2, Cyp6BJ1, Cyp6BQ17, Cyp6EG1, Cyp6EH1, Cyp6EJ1 Cyp4BN13v1 and Cyp4BN15, were highly expressed in cyhalothrin-treated larvae. These CYPs are the candidates that are involved in cyhalothrin detoxification. (C) 2013 Elsevier Inc. All rights reserved.

  • 相关文献

[1]Identification of carboxylesterase genes and their expression profiles in the Colorado potato beetle Leptinotarsa decemlineata treated with fipronil and cyhalothrin. Lu, Feng-Gong,Fu, Kai-Yun,Li, Qian,Li, Guo-Qing,Guo, Wen-Chao,Ahmat, Tursun.

[2]Insecticide resistance status of Colorado potato beetle (Coleoptera: Chrysomelidae) adults in Northern Xinjiang Uygur autonomous region. Jiang, Wei-Hua,Wang, Zhi-Tian,Xiong, Man-Hui,Lu, Wei-Ping,Liu, Ping,Li, Guo-Qing,Jiang, Wei-Hua,Wang, Zhi-Tian,Xiong, Man-Hui,Lu, Wei-Ping,Liu, Ping,Li, Guo-Qing,Guo, Wen-Chao.

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