文献类型: 外文期刊
作者: Liu, Zhiyang 1 ; Li, Xuejuan 1 ; Sun, Feng 1 ; Zhou, Tong 1 ; Zhou, Yijun 1 ;
作者机构: 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Jiangsu Tech Serv Ctr Diag & Detect Plant Virus D, Nanjing, Jiangsu, Peoples R China
2.Minist Agr, Sci Observat & Expt Stn Crop Pests Nanjing, Nanjing, Jiangsu, Peoples R China
3.Minist Agr, Sci Obse
关键词: Rice stripe virus;NS3;interaction;OsCIPK30;pathogenesis-related genes
期刊名称:FRONTIERS IN MICROBIOLOGY ( 影响因子:5.64; 五年影响因子:6.32 )
ISSN: 1664-302X
年卷期: 2017 年 8 卷
页码:
收录情况: SCI
摘要: Rice stripe virus (RSV) causes a severe disease in Oryza sativa (rice) in many Eastern Asian countries. The NS3 protein of RSV is a viral suppressor of RNA silencing, but plant host factors interacting with NS3 have not been reported yet. Here, we present evidence that expression of RSV NS3 in Arabidopsis thaliana causes developmental abnormalities. Through yeast two-hybrid screening and a luciferase complementation imaging assay, we demonstrate that RSV NS3 interacted with OsCIPK30, a CBL (calcineurin B-like proteins)-interaction protein kinase protein. Furthermore, OsCIPK30 was overexpressed to investigate the function of OsCIPK30 in rice. Our investigation showed that overexpression of OsCIPK30 in rice could delay the RSV symptoms and show milder RSV symptoms. In addition, the expression of pathogenesis-related genes was increased in OsCIPK30 transgenic rice. These results suggest that overexpression of OsCIPK30 positively regulates pathogenesis-related genes to enhance the tolerance to RSV in rice. Our findings provide new insight into the molecular mechanism underlying resistance to RSV disease.
- 相关文献
作者其他论文 更多>>
-
Investigation and characterization of rice dwarfing epidemic caused by southern rice black-streaked dwarf virus in Jiangsu in 2023
作者:Li, Chenyang;Yang, Wenxuan;Du, Linlin;Lin, Feng;Lan, Ying;Zhou, Tong;Yang, Wenxuan;Zhang, Yuanming;Qiu, Yanglin;Xu, Kai;Zhou, Tong;Zhu, Feng
关键词:Rice viral disease; SRBSDV; Jiangsu rice dwarfing epidemic; Virus detection; Koch's postulates
-
Development of a transgenic rice line with strong and broad resistance against four devastating rice viruses through expressing a single hairpin RNA construct
作者:Li, Chenyang;Wu, Jianxiang;Fu, Shuai;Xu, Yi;Wang, Yaqin;Zhou, Xueping;Li, Chenyang;Lan, Ying;Lin, Feng;Du, Linlin;Zhou, Tong;Yang, Xiuling;Zhou, Xueping
关键词:rice; resistance; virus; RNA interference; hairpin RNA transgene
-
Transgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant
作者:Latif, Muhammad Faizan;Tan, Jingquan;Zhang, Wang;Yang, Wenxuan;Zhuang, Tinghui;Lu, Wenlong;Qiu, Yanglin;Du, Xinying;Zhuang, Xinjian;Zhou, Tong;Yin, Jinlong;Xu, Kai;Zhou, Tong;Kundu, Jiban K.;Kundu, Jiban K.
关键词:MicroRNA; Potyvirus; SMV; P1 gene; Transgenic; Virus resistance
-
The miR-184-3p promotes rice black-streaked dwarf virus infection by suppressing Ken in Laodelphax striatellus (Fallén)
作者:Wu, Wei;Wang, Man;Deng, Zhiting;Xi, Minmin;Dong, Yan;Wang, Haitao;Zhang, Jianhua;Zhou, Yijun;Xu, Qiufang;Wu, Wei;Deng, Zhiting;Xi, Minmin;Wang, Changchun;Xu, Qiufang
关键词:miR-184-3p; Laodelphax striatellus; rice black-streaked dwarf virus; Ken; virus infection
-
Plasmodesmata-associated Flotillin positively regulates broad-spectrum virus cell-to-cell trafficking
作者:Ge, Panpan;Lu, Hong;Wang, Wei;Ma, Yonghuan;Cui, Feng;Ge, Panpan;Ma, Yonghuan;Cui, Feng;Li, Yi;Zhou, Tong;Wei, Taiyun;Wu, Jianguo
关键词:Flotillin; plasmodesmata-associated protein; rice; rice stripe virus; southern rice black-streaked dwarf virus; rice dwarf virus
-
Introgression of OsAP47 by marker-assisted selection enhanced resistance against southern rice black-streaked dwarf virus disease
作者:Lin, Feng;Zhang, Jiayuan;Sun, Yue;Du, Linlin;Li, Chenyang;Lan, Ying;Zhou, Tong;Lin, Feng;Zhang, Jiayuan;Sun, Yue;Jing, Dedao;Zhou, Tong
关键词:Rice; Marker-assisted selection; SRBSDVD; Resistance; Breeding
-
Comparative transcriptomics analysis of tolerant and sensitive genotypes reveals genes involved in the response to cold stress in bitter gourd (Momordica charantia L.)
作者:Ning, Yu;Liu, Zhiyang;Liu, Jing;Xu, Hai;Chen, Longzheng;Qi, Renjie;Xia, Pengfei;Yuan, Xihan
关键词: