Multipath colourimetric assay for copper(II) ions utilizing MarR functionalized gold nanoparticles
文献类型： 外文期刊
作者： Wang, Yulong 1 ; Wang, Limin 2 ; Su, Zhenhe 2 ; Xue, Juanjuan 2 ; Dong, Jinbo 2 ; Zhang, Cunzheng 1 ; Hua, Xiude 2 ; Wang 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Nanjing, Jiangsu, Peoples R China
3.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop D
期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）
ISSN： 2045-2322
年卷期： 2017 年 7 卷
页码：
收录情况： SCI
摘要： We use the multiple antibiotic resistance regulator (MarR), as a highly selective biorecognition elements in a multipath colourimetric sensing strategy for the fast detection of Cu2+ in water samples. The colourimetric assay is based on the aggregation of MarR-coated gold nanoparticles in the presence of Cu2+ ions, which induces a red-to-purple colour change of the solution. The colour variation in the gold nanoparticle aggregation process can be used for qualitative and quantitative detection of Cu2+ by the naked eye, and with UV-vis and smartphone-based approaches. The three analysis techniques used in the multipath colourimetric assay complement each other and provide greater flexibility for differing requirements and conditions, making the assay highly applicable for Cu2+ detection. Under optimal conditions, the Cu2+ concentration was quantified in less than 5 min with limits of detection for the naked eye, UV-vis and smartphone-based approaches of 1 mu M, 405 nM and 61 nM, respectively. Moreover, the sensing system exhibited excellent selectivity and practical application for Cu2+ detection in real water samples. Thus, our strategy has great potential for application in on-site monitoring of Cu2+, and the unique response of MarR towards copper ions may provide a new approach to Cu2+ sensing.
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