文献类型： 外文期刊
作者： Wang, Yulong 1 ; Wang, Limin 2 ; Xue, Juanjuan 2 ; Dong, Jinbo 2 ; Cai, Jia 2 ; Hua, Xiude 2 ; Wang, Minghua 2 ; Zhang, C 1 ;
作者机构： 1.Nanjing Agr Univ, Jiangsu Acad Agr Sci, Inst Plant Protect, Coll Plant Protect, Nanjing, Jiangsu, Peoples R China
2.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management Crop Dis & Pests, Nanjing, Jiangsu, Peoples R China
3.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Management
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2017 年 12 卷 1 期
页码：
收录情况： SCI
摘要： A signal-amplified lateral flow test strip (SA-LFTS) for the detection of Cu2+ in aqueous solution was constructed based on Cutcatalyzed click chemistry and hybridization of single stranded DNA (ssDNA). Alkyne and azide modified ssDNA acted as specific elements for Cu2+ recognition, and a chemical ligation product formed through Cutcatalyzed alkyneazide cycloaddition. Hybridization of ssDNA-labeled gold nanoparticles resulted in high sensitivity, and the output signal could be observed directly by the naked eye. Using the developed SA-LFTS under optimal conditions, Cu2+ could be detected rapidly with limit of detections of 5 nM and 4.2 nM by visual observation and quantitative analysis, respectively. The sensitivity (i.e. the visual limit of detection) of the SA-LFTS was 80-times higher than that of traditional LFTS. The SA-LFTS was applied to the determination of Cu2+ in municipal water and river water samples with the results showing good recovery and accuracy. The developed test strip is promising for point-of-care applications and detection of Cu2+ in the field.
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