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Identification of Aeromonas hydrophila Genes Preferentially Expressed after Phagocytosis by Tetrahymena and Involvement of Methionine Sulfoxide Reductases

文献类型： 外文期刊

作者： Pang, Maoda 1 ; Lin, Xiaoqin 1 ; Liu, Jin 1 ; Guo, Changming 1 ; Gao, Shanshan 1 ; Du, Hechao 1 ; Lu, Chengping 1 ; Liu, 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Dept Prevent Vet, Nanjing, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Food Safety, Key Lab Food Qual & Saftey Jiangsu Prov, State Key Lab Breeding Base, Nanjing, Jiangsu, Peoples R China

关键词： Aeromonas hydrophila;Tetrahymena;phagocytosis;SCOTS;msr genes

期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：5.293； 五年影响因子：5.882 ）

ISSN： 2235-2988

年卷期： 2016 年 6 卷

页码：

收录情况： SCI

摘要： Free-living protozoa affect the survival and virulence evolution of pathogens in the environment. In this study, we explored the fate of Aeromonas hydrophila when co-cultured with the bacteriovorous ciliate Tetrahymena thermophila and investigated bacterial gene expression associated with the co-culture. Virulent A. hydrophila strains were found to have ability to evade digestion in the vacuoles of this protozoan. In A. hydrophila, a total of 116 genes were identified as up-regulated following co-culture with T. thermophila by selective capture of transcribed sequences (SCOTS) and comparative dot-blot analysis. A large proportion of these genes (42/116) play a role in metabolism, and some of the genes have previously been characterized as required for bacterial survival and replication within macrophages. Then, we inactivated the genes encoding methionine sulfoxide reductases, msrA, and msrB, in A. hydrophila. Compared to the wild-type, the mutants Delta msrA and Delta msrAB displayed significantly reduced resistance to predation by T. thermophila, and 50% lethal dose (LD50) determinations in zebrafish demonstrated that both mutants were highly attenuated. This study forms a solid foundation for the study of mechanisms and implications of bacterial defenses.

  • 相关文献

[1]Identification of novel virulence-related genes in Aeromonas hydrophila by screening transposon mutants in a Tetrahymena infection model. Pang, Maoda,Xie, Xing,Dong, Yuhao,Du, Hechao,Wang, Nannan,Lu, Chengping,Liu, Yongjie,Pang, Maoda.

[2]Activation of macrophages by the ophiopogon polysaccharide liposome from the root tuber of Ophiopogon japonicus. Sun, Wenjing,Hu, Wenjie,Yang, Lumiao,Zhang, Weimin,Song, Xiaoping,Qu, Xiaohao,Zhang, Yueyang,Ma, Lin,Fan, Yunpeng,Meng, Kai.

[3]Catecholamine-Stimulated Growth of Aeromonas hydrophila Requires the TonB2 Energy Transduction System but Is Independent of the Amonabactin Siderophore. Dong, Yuhao,Liu, Jin,Pang, Maoda,Du, Hechao,Wang, Nannan,Awan, Furqan,Lu, Chengping,Liu, Yongjie,Pang, Maoda. 2016

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