您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development of real-time PCR assay for detection of porcine circovirus-like virus P1 in domestic pigs in China

文献类型： 外文期刊

作者： He, Kong-wang 1 ; Wen, Li-bin 2 ; Wang, Yong-shan 2 ; Lu, Cheng-ping 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Key Lab Vet Biol Engn & Technol, Natl Ctr Engn Res Vet Bioprod, Inst Vet Med,Minist Agr, Nanjing 210014, Jiangsu, Peoples R China

关键词： Porcine circovirus-like virus P1;PCV2;Fluorescence quantitative PCR;Melting curve

期刊名称：BMC VETERINARY RESEARCH （ 2020影响因子：2.741； 五年影响因子：2.955 ）

ISSN： 1746-6148

年卷期： 2015 年 11 卷

页码：

收录情况： SCI

摘要： Background: The porcine circovirus-like agent P1 is a newly discovered DNA virus with a single-stranded circular genome that is highly homologous to that of porcine circovirus type 2. P1 infection can cause symptoms resembling postweaning multisystemic wasting syndrome. This study aims to develop a rapid, sensitive and specific method to detect P1. Results: A pair of primers was designed and used to amplify a 119 bp DNA fragment to generate a recombinant plasmid which was served as the standard. A SYBR I qPCR protocol was established using the P1 recombinant plasmid standard and the sensitivity, specificity and stability of this method was analyzed. The results demonstrate a strong correlation with P1 recombinant plasmid titers when virus DNA copy numbers fall in between 10(0) similar to 10(9) copies/mu L. This method doesn't detect pseudo rabies, porcine parvovirus or porcine reproductive and respiratory syndrome virus; moreover it can distinguish porcine circovirus type 2 from P1 by melting temperature analysis. Coefficient of variation for each batch of reaction is less than 5 %. The serum virus titers of P1 positive in this study were measured by this protocol to be 103 to 107 copies/mL. Conclusions: The established qPCR is sensitive, specific, and reliable, which could be a useful tool when applied to quantification of P1 in a variety of samples from infected pigs.

  • 相关文献

[1]Molecular characterization of porcine circovirus-like virus P1 in eastern China. Wen, Libin,Jiao, Fangfang,Zhang, Dan,Mao, Aihua,Liu, Chuanmin,Xie, Jianping,He, Kongwang,Wen, Libin,Jiao, Fangfang,Zhang, Dan,Mao, Aihua,Liu, Chuanmin,Xie, Jianping,He, Kongwang,Li, Yiming.

[2]Development and application of an indirect enzyme-linked immunosorbent assay using recombinant truncated Cap protein for the diagnosis of porcine circovirus-like virus P1. Wen, Li-bin,Wen, Shi-fu,He, Kong-wang,Wen, Li-bin,Wen, Shi-fu,He, Kong-wang,Wen, Li-bin,Wen, Shi-fu,He, Kong-wang. 2016

[3]Porcine circovirus-like virus P1 in cattle, goats and rabbits in China. Wen, L.,Mao, A.,Fan, Z.,Li, W.,Xiao, Q.,Liu, Q.,Xie, J.,He, K.,Wen, L.,Mao, A.,Fan, Z.,Li, W.,Xiao, Q.,Liu, Q.,Xie, J.,He, K.. 2018

[4]Nitric oxide-generating compound GSNO suppresses porcine circovirus type 2 infection in vitro and in vivo. Liu, Chuanmin,Wen, Libin,Xiao, Qi,He, Kongwang,Liu, Chuanmin,Wen, Libin,Xiao, Qi,He, Kongwang,Liu, Chuanmin,Wen, Libin,Xiao, Qi,He, Kongwang,Liu, Chuanmin,Wen, Libin,Xiao, Qi,He, Kongwang. 2017

[5]Development of recombinant porcine parvovirus-like particles as an antigen carrier formed by the hybrid VP2 protein carrying immunoreactive epitope of porcine circovirus type 2. Pan, Qunxing,He, Kongwang,Pan, Qunxing,Huang, Kehe. 2008

[6]Role of Chinese herbal medicinal ingredients in secretion of cytokines by PCV2-induced endothelial cells. Hu, Yiyi,He, Kongwang,Wang, Xiaomin. 2016

[7]猪圆环病毒2型标签重组病毒构建和免疫试验. 华涛,唐波,常晨,刘国阳,张雪花,于漾,侯继波,张道华. 2018

[8]提高猪圆环病毒2型产量的方法比较. 华涛,张雪花,唐波,常晨,刘国阳,冯磊,吴培培,张道华,侯继波. 2015

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台