Improved oxidative tolerance in suspension-cultured cells of C-4-pepctransgenic rice by H2O2 and Ca2+ under PEG-6000
文献类型: 外文期刊
作者: Qian, Baoyun 1 ; Li, Xia 1 ; Liu, Xiaolong 1 ; Wang, Man 1 ;
作者机构: 1.China Natl Ctr Rice Improvement, Jiangsu High Qual Rice Res & Dev Ctr, Jiangsu Acad Agr Sci, Inst Food Crops,Nanjing Branch, Nanjing 210014, Peoples R China
2.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Jiangsu, Peoples R China
3.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Nanjing 210014, Peoples R China
4.Jiangsu
关键词: Calcium;drought;hydrogen peroxide;phosphoenolpyruvate carboxylase;rice (Oryza sativa L.)
期刊名称:JOURNAL OF INTEGRATIVE PLANT BIOLOGY ( 影响因子:7.061; 五年影响因子:6.002 )
ISSN: 1672-9072
年卷期: 2015 年 57 卷 6 期
页码:
收录情况: SCI
摘要: To understand the molecular responses of PC (Overexpressing the maize C-4-pepc gene, which encodes phosphoenolpyruvate carboxylase (PEPC)), to drought stress at cell level, we analyzed changes in the levels of signaling molecules (hydrogen peroxide (H2O2), calcium ion (Ca2+), and nitric oxide (NO)) in suspension-cultured PC and wild-type (WT) rice (Oryza sativa L.) cell under drought stress induced by 20% polyethylene glycol 6000 (PEG-6000). Results demonstrated that PC improved drought tolerance by enhancing antioxidant defense, retaining higher relative water content, survival percentages, and dry weight of cells. In addition, PEPC activity in PC under PEG treatment was strengthened by addition of H2O2 inhibitor, dimethylthiourea (DMTU) and NO synthesis inhibitor, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), respectively, while that in PC was weakened by addition of free calcium chelator, ethylene glycol-bis(b-aminoethylether)-N,N,N,N-tetraacetic acid (EGTA)+calcium channel outflow inhibitor, ruthenium red (RR)+plasma membrane channel blocker La(NO3)(3), but EGTA+RR did not. Results also showed that NO and Ca2+ was lying downstream of H2O2 in drought-induced signaling. Calcium ion was also involved in the expression of C-4-pepc in PC. These results suggested that PC could improve oxidative tolerance in suspension-cultured cells and the acquisition of this tolerance required downregulation of H2O2 and the entry of extracellular Ca2+ into cells across the plasma membrane for regulation of PEPC activity and C-4-pepc expression.
- 相关文献
作者其他论文 更多>>
-
CRISPR/Cas knockout of the NADPH oxidase gene OsRbohB reduces ROS overaccumulation and enhances heat stress tolerance in rice
作者:Liu, Xiaolong;Ji, Ping;Liao, Jingpeng;Duan, Ximiao;Luo, Zhiyang;Yang, Hongtao;Peng, Bo;Jiang, Kai;Yu, Xin;Jiang, Chang-Jie;Xu, Chen
关键词:Rice (
Oryza sativa L.); heat stress;OsRbohB -knockout; reactive oxygen species (ROS); grain yield -
Knockdown of tyrosine hydroxylase gene affects larval survival, pupation and adult eclosion in Plagiodera versicolora
作者:Liu, Xiaolong;Wang, Xin;Zhang, Qi;Lu, Min;Ze, Longji;Zhang, Hainan
关键词:cuticle tanning; mortality;
Plagiodera versicolora ; RNA interference; tyrosine hydroxylase -
Discovery of a novel Betacoronavirus 1, cpCoV, in goats in China: The new risk of cross-species transmission
作者:Mao, Li;Cai, Xuhang;Li, Jizong;Li, Xia;Li, Siyuan;Li, Wenliang;Li, Bin;Mao, Li;Cai, Xuhang;Li, Jizong;Li, Xia;Li, Siyuan;Li, Wenliang;Li, Bin;Mao, Li;Cai, Xuhang;Li, Jizong;Li, Xia;Li, Siyuan;Li, Wenliang;Li, Bin;Mao, Li;Cai, Xuhang;Li, Jizong;Li, Xia;Li, Siyuan;Li, Wenliang;Li, Bin;Mao, Li;Li, Jizong;Li, Xia;Li, Wenliang;Li, Bin;Cai, Xuhang;Li, Siyuan;Xu, Xingang;Lu, Honghui;Dong, Yichun;Zhai, Junjun
关键词:
-
Transcriptomics reveals the mechanism of action of ultrasonic treatment to promote mung bean(Vigna radiata L.) sprouting
作者:Lu, Ming;Xu, Yinghao;Wang, Man;Xu, Yinghao;Xu, Yinghao
关键词:Mung bean sprouts; Nutrient composition; Germination; Ultrasound; Transcriptomics
-
Genomic Characteristics and Pathogenicity of Novel Reassortant Mammalian Orthoreoviruses From Sheep, China
作者:Li, Xia;Cai, Xuhang;Li, Wenliang;Mao, Li;Li, Bin;Li, Xia;Cai, Xuhang;Li, Wenliang;Mao, Li;Li, Bin;Li, Xia;Cai, Xuhang;Li, Wenliang;Mao, Li;Li, Bin;Li, Xia;Cai, Xuhang;Li, Wenliang;Mao, Li;Li, Bin;Li, Xia;Li, Wenliang;Luo, Runbo;Suolang, Sizhu;Mao, Li;Li, Bin;Li, Xia;Cai, Xuhang;Li, Wenliang;Mao, Li;Li, Bin;He, Yi;Zhai, Junjun
关键词:mammalian orthoreoviruses (MRVs); pathogenicity; reassortment; sheep; virus isolation and identification
-
Response of Photosynthesis and Chlorophyll Fluorescence to Nitrogen Changes in Rice with Different Nitrogen Use Efficiencies
作者:Qi, Zexin;Tang, Rui;Zhang, Qiang;Sun, Wenzheng;Guan, Chenglong;Wang, Ye;Zhang, Mengru;Ding, Jiale;Zhang, Yuankai;Yang, Hong;Yang, Ying;Zhang, Zhian;Ling, Fenglou;Xu, Chen;Liu, Xiaolong
关键词:rice; chlorophyll; chlorophyll fluorescence; low nitrogen tolerance; photosynthesis
-
Improvement in Nitrogen-Use Efficiency Increases Salt Stress Tolerance in Rice Seedlings and Grain Yield in Salinized Soil
作者:Ji, Ping;Li, Xingjie;Chen, Yunfeng;Liu, Xiaolong;Wang, Jinze;Luo, Zhiyang;Cheng, Ziwen;Chen, Jianrui;Xu, Chen;Ling, Fenglou;Qi, Zexin;Zhang, Zhian
关键词:rice (
Oryza sativa L.); salt stress; nitrogen-use efficiency; grain yield



