Optimization on Preparation Conditions of Salidroside Liposome and Its Immunological Activity on PCV-2 in Mice
文献类型： 外文期刊
作者： Feng, Yibo 1 ; Zhao, Xiaojuan 2 ; Lv, Fang 2 ; Zhang, Jinqiu 2 ; Deng, Bihua 2 ; Zhao, Yanhong 3 ; Hu, Yuanliang 1 ; Wang 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Inst Tradit Chinese Vet Med, Nanjing 210095, Jiangsu, Peoples R China
2.Jiangsu Acad Agr Sci, Natl Res Ctr Vet Biol Engn & Technol, Nanjing 210014, Peoples R China
3.Jiangsu Acad Agr Sci, Natl Res Ctr
期刊名称：EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE （ 影响因子：2.629； 五年影响因子：2.845 ）
ISSN： 1741-427X
年卷期： 2015 年
页码：
收录情况： SCI
摘要： The aim of this study was to optimize the preparation conditions of salidroside liposome with high encapsulation efficiency (EE) and to study the immunological enhancement activity of salidroside liposome as porcine circovirus type 2 virus (PCV-2) vaccine adjuvant. Response surface methodology (RSM) was selected to optimize the conditions for the preparation of salidroside liposome using Design-Expert V8.0.6 software. Three kinds of salidroside liposome adjuvants were prepared to study their adjuvant activity. BALB/c mice were immunized with PCV-2 encapsulated in different kinds of salidroside liposome adjuvants. The PCV-2-specific IgG in immunized mice serum was determined with ELISA. The results showed that when the concentration of ammonium sulfate was 0.26mol.L-1, ethanol volume 6.5 mL, temperature 43 degrees C, ethanol injection rate 3mL.min(-1), and salidroside liposome could be prepared with high encapsulation efficiency of 94.527%. Salidroside liposome as adjuvant could rapidly induce the production of PCV-2-specific IgG and salidroside liposome I adjuvant proved to provide the best effect among the three kinds of salidroside liposome adjuvants.
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